FtsZ Constriction Force - Curved Protofilaments Bending Membranes

Sub-cellular Biochemistry
Harold P Erickson, Masaki Osawa

Abstract

FtsZ assembles in vitro into protofilaments (pfs) that are one subunit thick and ~50 subunits long. In vivo these pfs assemble further into the Z ring, which, along with accessory division proteins, constricts to divide the cell. We have reconstituted Z rings in liposomes in vitro, using pure FtsZ that was modified with a membrane targeting sequence to directly bind the membrane. This FtsZ-mts assembled Z rings and constricted the liposomes without any accessory proteins. We proposed that the force for constriction was generated by a conformational change from straight to curved pfs. Evidence supporting this mechanism came from switching the membrane tether to the opposite side of the pf. These switched-tether pfs assembled "inside-out" Z rings, and squeezed the liposomes from the outside, as expected for the bending model. We propose three steps for the full process of cytokinesis: (a) pf bending generates a constriction force on the inner membrane, but the rigid peptidoglycan wall initially prevents any invagination; (b) downstream proteins associate to the Z ring and remodel the peptidoglycan, permitting it to follow the constricting FtsZ to a diameter of ~250 nm; the final steps of closure of the septum and membrane fusion ...Continue Reading

Citations

Jul 13, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Harold P Erickson
Feb 16, 2018·Frontiers in Microbiology·Masaki Osawa, Harold P Erickson
Apr 4, 2018·The Journal of Biological Chemistry·Haiyan HuangYaodong Chen
Oct 31, 2018·FEMS Microbiology Reviews·Pablo Mateos-GilMarisela Vélez
Jul 11, 2020·Advanced Biosystems·Daniel HürtgenPetra Schwille
Mar 17, 2020·The Journal of General and Applied Microbiology·Ayumi Kizawa, Takashi Osanai
Oct 27, 2020·Computational and Structural Biotechnology Journal·Pouyan AsgharzadehOliver Röhrle

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