PMID: 22362029Mar 1, 2012Paper

Fub1p, a novel protein isolated by boundary screening, binds the proteasome complex

Genes & Genetic Systems
Akira HatanakaMasaya Oki

Abstract

Silenced chromatin domains are restricted to specific regions. Eukaryotic chromosomes are organized into discrete domains delimited by domain boundaries. From approximately 6,000 genes in Saccharomyces cerevisiae, we previously isolated 55 boundary genes. In this study, we focus on the molecular function of one of boundary genes, YCR076C/FUB1 (function of boundary), whose function has not been clearly defined in vivo. Biochemical analysis of Fub1p revealed that it interacted with multiple subunits of the 20S proteasome core particle (20S CP). To further clarify the functional link between Fub1p and proteasome, several proteasome mutants were analyzed. Although only 20S CP subunits were isolated as Fub1p interactors, a genetic interaction was also observed for component of 19S regulatory particle (19S RP) suggesting involvement of Fub1p with the whole proteasome. We also analyzed the mechanism of boundary establishment by using proteasome composition factor-deficient strains. Deletion of pre9 and ump1, whose products have effects on the 20S CP, resulted in a decrease in boundary function. Domain analyses of Fub1p identified a minimum functional domain in the C terminus that was essential for boundary establishment and showed a l...Continue Reading

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Citations

Jul 4, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuma KamataMasaya Oki
Mar 10, 2016·Journal of Biochemistry·Risa MitsumoriMasaya Oki
May 7, 2015·Genome Biology and Evolution·Philippe FortOlivier Coux
Sep 21, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuma KamataMasaya Oki
Jun 16, 2016·Genes & Genetic Systems·Risa MitsumoriMasaya Oki
Oct 22, 2014·Molecular and Cellular Biology·Hideki YashirodaShigeo Murata
Jun 25, 2019·Frontiers in Molecular Biosciences·Richard S Marshall, Richard D Vierstra

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