Fucoidan in a 3D scaffold interacts with vascular endothelial growth factor and promotes neovascularization in mice

Drug Delivery and Translational Research
Agung PurnamaCatherine Le Visage

Abstract

The aim of this study was to functionalize 3D porous cross-linked scaffolds with natural non-animal sulfated polysaccharide fucoidans in order to allow a delivery of vascular endothelial growth factor (VEGF) and potentiate its angiogenic activity. Microporous (20 μm) and macroporous (200 μm) scaffolds were functionalized with low, medium, or high molecular weight fucoidans (named LMWF, MMWF, and HMWF, respectively). In vitro, addition of fucoidans promoted endothelial progenitor cells proliferation in both micro- and macroporous scaffolds. While control scaffolds without fucoidans loaded with VEGF165 (100 ng) showed a fast burst release in PBS during the first 24 h, MMWF significantly reduced the VEGF165 release (p < 0.001). Surface plasmon resonance experiments confirmed a direct interaction between MMWF and VEGF165, characterized by an affinity K D (K d/K a) of 1 × 10(-9) M. In a subcutaneous angiogenesis model in mice, fucoidan functionalized scaffolds showed a more intense vascularization response than control groups. Expression of isolectin-B4 and α-smooth muscle actin, as well as confinement of erythrocytes, demonstrated the neoformed blood vessels functionality. There was a significant difference in neovessel area and ne...Continue Reading

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Citations

Aug 3, 2016·Marine Drugs·Lucas CholletFrédéric Chaubet
Aug 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xian Li, Xiulan Su
Dec 30, 2017·Macromolecular Bioscience·Byeong-Ju KwonHak-Joon Sung
May 26, 2018·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Valérie Metzinger-Le MeuthLaurent Metzinger
Aug 8, 2019·Marine Drugs·Aleksandra CitkowskaKatarzyna Winnicka
Jun 19, 2019·Marine Drugs·Marisol Zuluaga TamayoGraciela Pavon-Djavid
May 28, 2020·International Journal of Molecular Sciences·Marie-Noëlle LabourDidier Letourneur
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Teresa Simon-YarzaDidier Letourneur
Mar 13, 2021·Advanced Therapeutics·Carlisle R DeJuliusCraig L Duvall
May 25, 2020·International Journal of Biological Macromolecules·Ramar ManikandanNarayanasamy Marimuthu Prabhu

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