Fucosyltransferase IV (FUT4) as an effective biomarker for the diagnosis of breast cancer

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Xiaomei YanQiu Yan

Abstract

Specific enzymes are involved in altered glycosylation of cancer. Fucosyltransferase IV (FUT4) is associated with the proliferation and metastasis of breast cancer. The application of FUT4 assay in the serum has not been reported yet. Here, the expression level of FUT4 in the breast cancer patient's tissues (n=60) was analyzed by immunohistochemistry (IHC) and the secreted FUT4 in blood serum samples (n=225) was detected by enzyme-linked immunosorbent assay (ELISA). Using low metastatic MCF-7 and high metastatic MDA-MB-231 breast cancer cell lines, FUT4 expression was also detected by reverse transcription-polymerase chain reaction (RT-PCR), Western blot and immunofluorescent staining. The conventional cancer biomarkers cancer antigen (CA15.3) and carcinoembryonic antigen (CEA) was analyzed by Elecsys-electrochemical immune assay (ECLIA) to compare specificity and sensitivity with that of FUT4. We have observed a significant high expression of FUT4 in breast cancer tissues and serums as compared to the normal tissues (P<0.01) and control serums (P<0.05). FUT4 expression was increased in MDA-MB-231 cells vs. that in MCF-7 cells. Furthermore, the results of receiver operating characteristic (ROC) analysis was shown, area under cu...Continue Reading

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Citations

Oct 3, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Faisal AzizQiu Yan
Sep 1, 2015·Experimental and Molecular Pathology·Jessica Catarine Frutuoso do NascimentoEduardo Isidoro Carneiro Beltrão
Nov 4, 2015·Frontiers in Oncology·Alexandra C KölblUdo Jeschke
Apr 15, 2016·Journal of the National Cancer Institute·Takahiro OsugaJunji Kato
Sep 16, 2016·Journal of the National Cancer Institute·Takahiro OsugaJunji Kato
Dec 16, 2016·Glycoconjugate Journal·Bhairavi N Vajaria, Prabhudas S Patel
Oct 26, 2018·Journal of Cancer Research and Clinical Oncology·Chang LiuXiujuan Qu
Aug 4, 2019·Pharmaceuticals·Maryam NakhjavaniJennifer E Hardingham
Jan 22, 2020·Journal of Cellular and Molecular Medicine·Minzi DengHeli Liu
Dec 13, 2019·Cellular Oncology (Dordrecht)·Meysam YousefiSeyed H Ghaffari
Oct 23, 2018·Oncotarget·Hector A CuelloMariano R Gabri
Jan 9, 2019·Frontiers in Oncology·Liyuan JiaJihong Cui
Jul 24, 2020·Sensors·Veronika GajdosovaJan Tkac
Nov 7, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Divya ThomasPrakash Radhakrishnan
Mar 11, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Huimin LiuDingkun Zhang

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