PMID: 11320063Apr 26, 2001Paper

Fucosyltransferases in Schistosoma mansoni development

Glycobiology
E T MarquesR L Schnaar

Abstract

Glycoconjugate-bound fucose, abundant in the parasite Schistosoma mansoni, has been found in the form of Fucalpha1,3GlcNAc, Fucalpha1,2Fuc, Fucalpha1,6GlcNAc, and perhaps Fucalpha1,4GlcNAc linkages. Here we quantify fucosyltransferase activities in three developmental stages of S. mansoni. Assays were performed using fluorophore-assisted carbohydrate electrophoresis with detection of radioactive fucose incorporation from GDP-[(14)C]-fucose into structurally defined acceptors. The total fucosyltransferase-specific activity in egg extracts was 50-fold higher than that in the other life stages tested (cercaria and adult worms). A fucosyltransferase was detected that transferred fucose to type-2 oligosaccharides (Galbeta1,4GlcNAc-R), both sialylated (with the sialic acid attached to the terminal Gal by alpha2,3 or 2,6 linkage) and nonsialylated. Another fucosyltransferase was identified that transferred fucose to lactose-based and type-2 fucosylated oligosaccharides, such as LNFIII (Galbeta1,4(Fucalpha1,3)GlcNAcbeta1,3Galbeta1,4Glc). A low level of fucosyltransferase that transfers fucose to no-sialylated type-1 oligosaccharides (Galbeta1,3GlcNAc-R) was also detected. These studies revealed multifucosylated products of the reaction...Continue Reading

References

Aug 4, 1995·The Journal of Biological Chemistry·K Y DoR D Cummings
Jan 1, 1996·Experimental Parasitology·R DeBose-BoydR D Cummings
Jun 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R D Cummings, A K Nyame
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Apr 26, 2000·Molecular and Biochemical Parasitology·F TrotteinM Capron

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Citations

Nov 4, 2009·PLoS Neglected Tropical Diseases·Jennifer M FitzpatrickKarl F Hoffmann
Jun 13, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Erika Staudacher
Nov 20, 2015·Glycobiology·Megan L MickumRichard D Cummings
Apr 10, 2013·Chembiochem : a European Journal of Chemical Biology·Sonia SernaNiels-Christian Reichardt
Jun 7, 2003·Parasite Immunology·Francisca MutapiPatricia D Ndhlovu
Mar 6, 2007·Trends in Parasitology·Cornelis H HokkeKarl F Hoffmann
Dec 18, 2004·Glycobiology·Katharina PaschingerIain B H Wilson
Sep 16, 2006·Glycobiology·Bing MaDiane E Taylor
Apr 17, 2013·Chemical Society Reviews·Zhijay TuChun-Hung Lin
Apr 22, 2021·Organic & Biomolecular Chemistry·Irene Conforti, Alberto Marra

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