Full Expression of Cardiomyopathy Is Partly Dependent on B-Cells: A Pathway That Involves Cytokine Activation, Immunoglobulin Deposition, and Activation of Apoptosis

Journal of the American Heart Association
Andrea M Cordero-ReyesGuillermo Torre-Amione

Abstract

Limited information exists on the role of B-cell-dependent mechanisms in the progression of heart failure (HF). However, in failing human myocardium, there is evidence of deposition of activated complement components as well as anticardiac antibodies. We aimed to determine the contribution of B-cells in HF progression using a nonsurgical mouse model of nonischemic cardiomyopathy (CMP). CMP protocol involved the use of l-NAME and NaCl in the drinking water and angiotensin-II infusion for 35 days. At day 35, mice were analyzed by cardiac magnetic resonance imaging, gene expression, and histology. Mice (12 weeks old) were divided into 4 groups, all in C57BL/6 background: wild-type (WT) CMP; severe combined immunodeficiency (SCID) CMP (T- and B-cell deficient); CD22(-) CMP (B-cell depleted); and Nude CMP (T-cell deficient), with their respective controls. We performed B-cell depletion and reconstitution protocols. The protective effect of B-cell depletion was demonstrated by a significant reduction of cell hypertrophy and collagen deposition and a preserved ejection fraction in the CD22(-) CMP group compared to WT CMP. Once SCID mice underwent B-cell reconstitution with isolated CMP B-cells, the CMP phenotype was restored. Furtherm...Continue Reading

References

Apr 11, 2001·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·B A BrucknerG Torre-Amione
Jul 13, 2001·European Journal of Pharmacology·A StaudtS B Felix
Apr 8, 2003·European Journal of Pharmacology·Yvonne StaudtAlexander Staudt
Nov 11, 2003·European Journal of Heart Failure·Thomas PeschelJosef Niebauer
Apr 20, 2005·International Journal of Cardiology·Hiroshi SuzukiTakashi Katagiri
May 27, 2006·The American Journal of Cardiology·Guilherme H M OliveiraGuillermo Torre-Amione
Apr 18, 2008·Current Opinion in Immunology·Frances E Lund
Apr 29, 2009·European Journal of Clinical Investigation·J Latva-HirveläP Saukko
Sep 28, 2010·Journal of Clinical Apheresis·Guillermo Torre-AmioneJerry D Estep
Oct 20, 2010·Basic Research in Cardiology·Andreas O DoeschHugo A Katus
May 24, 2014·Circulation Research·Dimitrios TsiantoulasChristoph J Binder

❮ Previous
Next ❯

Citations

Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Uros KuzmanovAndrew Emili
Jun 7, 2018·Physiological Reviews·Steven J ForresterSatoru Eguchi
Aug 30, 2018·Physiological Reviews·Vanessa Frodermann, Matthias Nahrendorf
Sep 16, 2017·Circulation Research·Barry H Trachtenberg, Joshua M Hare
Jul 19, 2019·Circulation Research·Kenneth M BorowEmerson C Perin
Aug 16, 2019·European Journal of Preventive Cardiology·Alberto AimoAlberto Giannoni
Sep 27, 2019·Journal of Cellular and Molecular Medicine·Patricia van den HoogenJoost P G Sluijter
Jun 14, 2020·ESC Heart Failure·Gerardo García-RivasGuillermo Torre-Amione
May 23, 2020·Journal of Cardiovascular Translational Research·Ahmed ElsanhourySophie Van Linthout
Apr 26, 2017·Frontiers in Endocrinology·Paras K MishraSushil K Mahata
Apr 29, 2020·Annual Review of Immunology·Luigi AdamoDouglas L Mann
Sep 11, 2020·Current Cardiology Reports·Elena C CastilloGuillermo Torre-Amione
May 15, 2019·International Journal of Molecular Sciences·Diana Michels da SilvaTobias Graf
Jan 10, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Dale J HamiltonAnisha A Gupte
Apr 24, 2020·Frontiers in Physiology·Ama Dedo Okyere, Douglas G Tilley
Oct 13, 2020·Frontiers in Physiology·Toby P Thomas, Laurel A Grisanti
Feb 16, 2021·Frontiers in Genetics·Guangyu WangArvind Bhimaraj
Jan 24, 2020·Nature Reviews. Cardiology·Luigi AdamoDouglas L Mann
Jun 8, 2021·Cardiovascular Research·Marida Sansonetti, Leon J De Windt
Aug 13, 2021·Aging Cell·Thassio MesquitaAhmed Ibrahim
Jul 9, 2021·Journal of Veterinary Internal Medicine·Natalia DruzhaevaAleksandra Domanjko Petrič

❮ Previous
Next ❯

Methods Mentioned

BETA
antibody assay
PCR
electrophoresis
FCS
genetic modifications

Software Mentioned

Image Pro Plus
GraphPad Prism
GraphPad
FACSDiva

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

Cardiac Remodeling

Cardiac remodeling in response to a myocardial infarction is characterized by progressive ventricular dilatation, cardiac hypertrophy, fibrosis, and deterioration of cardiac performance. Discover the latest research on Cardiac Remodeling here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis