Full-length cDNA sequences from Rhesus monkey placenta tissue: analysis and utility for comparative mapping.

BMC Genomics
Dae-Soo KimKyu-Tae Chang

Abstract

Rhesus monkeys (Macaca mulatta) are widely-used as experimental animals in biomedical research and are closely related to other laboratory macaques, such as cynomolgus monkeys (Macaca fascicularis), and to humans, sharing a last common ancestor from about 25 million years ago. Although rhesus monkeys have been studied extensively under field and laboratory conditions, research has been limited by the lack of genetic resources. The present study generated placenta full-length cDNA libraries, characterized the resulting expressed sequence tags, and described their utility for comparative mapping with human RefSeq mRNA transcripts. From rhesus monkey placenta full-length cDNA libraries, 2000 full-length cDNA sequences were determined and 1835 rhesus placenta cDNA sequences longer than 100 bp were collected. These sequences were annotated based on homology to human genes. Homology search against human RefSeq mRNAs revealed that our collection included the sequences of 1462 putative rhesus monkey genes. Moreover, we identified 207 genes containing exon alterations in the coding region and the untranslated region of rhesus monkey transcripts, despite the highly conserved structure of the coding regions. Approximately 10% (187) of all...Continue Reading

References

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Citations

Nov 22, 2014·Alternatives to Laboratory Animals : ATLA·Jarrod Bailey

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Datasets Mentioned

BETA
FS722297-FS724151

Methods Mentioned

BETA
cDNA library construction

Software Mentioned

BLAST
RepeatMasker
BLASTN
Repbase Update

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