Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria.

Virology Journal
Abdelheq BarberisMariette F Ducatez

Abstract

H9N2 avian influenza viruses (AIV) has a worldwide geographic distribution and affects poultry of different types of production. H9N2 AIV was first reported in the Northeast of Algeria in April 2017, following an outbreak associated with high mortality, in broiler flocks. In the present study, we report full-length genome sequences of AIV H9N2, and the detailed phylogeny and molecular genetic analyses. Ten AIV H9N2 strains, collected in broiler flocks, were amplified in 9-day-old embryonated specific pathogen free (SPF) chicken eggs. Their full-length genomes were successfully sequenced and phylogenetic and molecular characterizations were conducted. Phylogenetic analysis showed that the isolates were monophyletic, grouped within the G-1 lineage and were very close to Moroccan and Algerian strains identified in 2016 and 2017, respectively. The low pathogenicity of the strains was confirmed by the sequence motif (335RSSR/GLF341) at the hemagglutinin (HA) cleavage site. An exclusive substitution (T197A) that had not been previously reported for H9N2 viruses; but, conserved in some pandemic H1N1 viruses, was observed. When compared to the G1-like H9N2 prototype, the studied strains showed one less glycosylation site in HA, but 2-3...Continue Reading

References

Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·J N VargheseJ L McKimm-Breschkin
Jun 17, 1998·Journal of Virology·S K BiswasD P Nayak
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y P LinA Hay
Oct 24, 2000·Journal of Virology·J M KatzK Subbarao
Jan 29, 2002·Archives of Virology·E HoffmannD R Perez
Jun 26, 2003·Nucleic Acids Research·Ramu ChennaJulie D Thompson
Oct 25, 2003·Avian Diseases·H Nili, K Asasi
Feb 15, 2005·Journal of Virology·Ron A M FouchierAlbert D M E Osterhaus
Dec 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·G GabrielJ Stech
Sep 15, 2006·Journal of Virology·Zejun LiHualan Chen
Nov 1, 2006·Emerging Infectious Diseases·Guang-Wu ChenShin-Ru Shih
Jul 27, 2007·Journal of Virology·David B FinkelsteinClayton W Naeve
Feb 1, 2008·Nature·Jason R Schnell, James J Chou
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·David JacksonRobert A Lamb
Sep 13, 2008·Annual Review of Microbiology·Nadia NaffakhSylvie van der Werf
Dec 18, 2009·The Journal of General Virology·Jin Hyun KimYoshihiro Kawaoka
Jul 31, 2013·Influenza and Other Respiratory Viruses·Ulrich WerneryRobert G Webster
Sep 27, 2013·Influenza and Other Respiratory Viruses·Aurora Romero-Tejeda, Ilaria Capua

❮ Previous
Next ❯

Citations

Nov 9, 2021·Frontiers in Veterinary Science·Xueyun LiYuelong Shu

❮ Previous
Next ❯

Datasets Mentioned

BETA
MK796882
MK796883
MK796884
MK796885
MK796886
MK796887
MK796868
MK796869
MK796870
MK796871

Methods Mentioned

BETA
PCR
glycosylation

Software Mentioned

Blast
BioEdit Sequence Alignment Editor
MEGA X
DNA Baser
ClustalW

Related Concepts