Full length ryanodine receptor subtype 3 encodes spontaneous calcium oscillations in native duodenal smooth muscle cells

Cell Calcium
Fabrice DabertrandJ L Morel

Abstract

Two isoforms of the ryanodine receptor subtype 3 (RYR3) have been described in smooth muscle. The RYR3 short isoform (RYR3S) negatively regulates the calcium-induced calcium release mechanism encoded by the RYR2, whereas the role of the full length isoform of RYR3 (RYR3L) was still unclear. Here, we describe RYR-dependent spontaneous Ca(2+) oscillations measured in 10% of native duodenum myocytes. We investigated the role of RYR3 isoforms in these spontaneous Ca(2+) signals. Inhibition of RYR3S expression by antisense oligonucleotides revealed that both RYR2 and RYR3L were able to propagate spontaneous Ca(2+) waves that were distinguishable by frequency analysis. When RYR3L expression was inhibited, the spontaneous Ca(2+) oscillations were never observed, indicating that RYR3S inhibited the function of RYR2. RYR2 expression inhibition led to Ca(2+) oscillations identical to those observed in control cells suggesting that RYR3S did not functionally interact with RYR3L. The presence and frequency of RYR3L-dependent Ca(2+) oscillations were dependent on sarcoplasmic reticulum Ca(2+) content as revealed by long-term changes of the extracellular Ca(2+) concentration. Our study shows that, in native duodenal myocytes, the spontaneous...Continue Reading

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Citations

Dec 15, 2012·Pflügers Archiv : European journal of physiology·Carole Georgeon-ChartierJean-Luc Morel
Dec 12, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Fabrice DabertrandJoseph E Brayden
Dec 17, 2014·European Journal of Pharmacology·Raphael BoursereauJean-Luc Morel
May 16, 2009·Journal of Pharmacological Sciences·Akitoshi OhnoYuji Imaizumi
Mar 15, 2018·American Journal of Physiology. Cell Physiology·Katsuhito MatsukiYuji Imaizumi
Jul 3, 2019·Pediatric Surgery International·Anne Marie O' DonnellPrem Puri
Dec 17, 2017·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Masayo KoideFabrice Dabertrand
Nov 21, 2016·Pflügers Archiv : European journal of physiology·Katsuhito MatsukiYuji Imaizumi

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