Full physiological maturation in vitro of immature mouse oocytes induced by sequential treatment with follicle-stimulating hormone and luteinizing hormone

Journal of in Vitro Fertilization and Embryo Transfer : IVF
M JinnoG D Hodgen

Abstract

Cumulus cell-enclosed immature mouse oocytes were matured in medium supplemented with various combinations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol. FSH or LH alone stimulated oocyte maturation, resulting in a significant increase in the rate of development to blastocysts following fertilization in vitro and embryo culture. There was no significant difference between FSH and LH. The effect of FSH was neutralized by FSH antiserum, while that of LH was not, indicating that the stimulation of maturation by LH was not due to FSH contamination in the LH preparation. When LH was added after 2 hr of culture with FSH (sequential combination), blastocyst development was significantly increased compared with FSH alone, reaching the same level as the in vivo matured oocytes. The addition of estradiol, 0.1 ng/ml, to the sequential combination of FSH and LH had no effect, while 0.01 and 1 ng/ml produced a negative effect. The birth rate of normal live offspring following embryo transfer showed no significant difference between embryos derived from oocytes matured in vivo and in vitro (sequential combination with or without 0.1 ng/ml estradiol) or between the two in vitro treatment groups.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N Dekel, W H Beers
Mar 1, 1979·Journal of Reproduction and Fertility·R ShalgiP F Kraicer
Feb 1, 1989·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M JinnoG D Hodgen
Dec 1, 1987·Fertility and Sterility·M F HayesK S Moghissi
Mar 1, 1987·Biology of Reproduction·M L Leibfried-RutledgeN L First
Jan 1, 1985·Annals of the New York Academy of Sciences·L L Veeck
Jun 1, 1983·The Journal of Experimental Zoology·M L Leibfried, B D Bavister
Apr 1, 1984·Developmental Biology·A C Schroeder, J J Eppig

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Citations

Apr 1, 1997·Journal of Assisted Reproduction and Genetics·K L DurinziS E Lanzendorf
Dec 1, 1992·Molecular Reproduction and Development·C BruckerG D Hodgen
Dec 8, 2010·Proteomics. Clinical Applications·Michael D PowellPeter Sutovsky
Mar 1, 2000·Molecular Reproduction and Development·A E BaltarM T Catanho

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