Full-term development of gaur-bovine interspecies somatic cell nuclear transfer embryos: effect of trichostatin A treatment

Cellular Reprogramming
Kanokwan SrirattanaRangsun Parnpai

Abstract

Trichostatin A (TSA) has previously been used in somatic cell nuclear transfer (SCNT) to improve the cloning efficiency in several species, which led our team to investigate the effects of TSA on the full-term development of bovine SCNT and gaur-bovine interspecies SCNT (gaur iSCNT; gaur somatic cells as donors and bovine oocytes as recipients) embryos. Treatment with 50 nM TSA for 10 h after fusion had no positive effects on the rates of fusion, cleavage, or the development to eight-cell or morula stages in both bovine SCNT and gaur iSCNT embryos. However, TSA treatment significantly enhanced the blastocyst formation rate in bovine SCNT embryos (44 vs. 32-34% in the TSA-treated and TSA-untreated groups, respectively), but had no effects on gaur iSCNT embryos. The fresh blastocysts derived from bovine SCNT and gaur iSCNT embryos (fresh groups), as well as vitrified bovine SCNT blastocysts (vitrified group), were transferred to bovine recipients. We found that TSA treatment increased the pregnancy rates only in recipients receiving fresh bovine SCNT embryos. In recipients receiving TSA-treated bovine SCNT embryos, three cloned calves from the fresh group and twin cloned calves from the vitrified group were delivered; however, no...Continue Reading

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Citations

Sep 17, 2013·Cellular Reprogramming·Irina LagutinaCesare Galli
Jun 5, 2013·Zygote : the Biology of Gametes and Early Embryos·Li-Bing MaTeng Cheng
May 5, 2016·Zoo Biology·Joseph SaragustyThomas B Hildebrandt
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Nov 21, 2017·Journal of Animal Science and Technology·A CordovaG F Mastromonaco

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Methods Mentioned

BETA
fluorescence microscopy
genotyping
PCR
cesarean section
acetylation

Software Mentioned

Statistical Analysis System ( SAS

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