Full-thickness porcine burns infected with Staphylococcus aureus or Pseudomonas aeruginosa can be effectively treated with topical antibiotics

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
David M TsaiE J Caterson

Abstract

Burn and blast injuries are frequently complicated by invasive infections, which lead to poor wound healing, delay in treatment, disability, or death. Traditional approach centers on early debridement, fluid resuscitation, and adjunct intravenous antibiotics. These modalities often prove inadequate in burns, where compromised local vasculature limits the tissue penetration of systemic antibiotics. Here, we demonstrate the treatment of infected burns with topical delivery of ultrahigh concentrations of antibiotics. Standardized burns were inoculated with Staphylococcus aureus or Pseudomonas aeruginosa. After debridement, burns were treated with either gentamicin (2 mg/mL) or minocycline (1 mg/mL) at concentrations greater than 1,000 times the minimum inhibitory concentration. Amount of bacteria was quantified in tissue biopsies and wound fluid following treatment. After six days of gentamicin or minocycline treatment, S. aureus counts decreased from 4.2 to 0.31 and 0.72 log CFU/g in tissue, respectively. Similarly, P. aeruginosa counts decreased from 2.5 to 0.0 and 1.5 log CFU/g in tissue, respectively. Counts of both S. aureus and P. aeruginosa remained at a baseline of 0.0 log CFU/mL in wound fluid for both treatment groups. T...Continue Reading

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Citations

Jul 1, 2017·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Melissa A OleksonRodney K Chan
Nov 1, 2018·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kristo NuutilaElof Eriksson
Dec 5, 2019·Annals of Clinical Microbiology and Antimicrobials·Gabrielle S DijksteelBouke K H L Boekema
Jun 23, 2018·Journal of Burn Care & Research : Official Publication of the American Burn Association·Lu YangElof Eriksson
Jun 30, 2019·Journal of Burn Care & Research : Official Publication of the American Burn Association·Joshua M GrolmanKristo Nuutila
Nov 18, 2020·Translational Vision Science & Technology·Jennifer S McDanielGina L Griffith

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