Fullerenol C60 (OH)36 protects human erythrocyte membrane against high-energy electrons

Biochimica Et Biophysica Acta. Biomembranes
Jacek GrebowskiAnita Krokosz

Abstract

Fullerenols (polyhydroxylated fullerene C60) are nanomaterial with potentially broad applicability in biomedical sciences with high antioxidant ability, thus, we investigated the radioprotecting potential of fullerenol C60(OH)36 on human erythrocytes irradiated by high-energy electrons of 6 MeV. The results demonstrate that C60(OH)36 at concentration of 150 μg/mL protects the erythrocytes against the radiation-induced hemolysis (comparing to non-protected cells, we observed 30% and 39% protection for 0.65 and 1.3 kGy irradiation doses, respectively). The protecting effect was confirmed by 32% decreased release of potassium cations comparing to the cells irradiated without C60(OH)36. Measurements of the amount of lactate dehydrogenase (LDH) released from the irradiated erythrocytes showed that the size of the pores formed by irradiation was not sufficient to release LDH across the erythrocyte membranes. We also report a significant decrease of the affinity of acetylcholinesterase (AChE) for the substrate in the presence of fullerenol, indicating the relatively strong adsorption of C60(OH)36 to components of plasma membrane. Changes in membrane fluidity detected by fluorescence spectroscopy and conformational changes in membrane ...Continue Reading

Citations

Sep 5, 2019·International Journal of Molecular Sciences·Chiang-Wen LeeChian-Hui Lai
Nov 20, 2019·Journal of Biomedical Materials Research. Part a·Xiliu ZhangDongsheng Yu
Aug 29, 2018·Scientific Reports·Tihomir KovačBojan Šarkanj
Mar 25, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Fahad ShafiqMuhammad Ali
Feb 26, 2019·Oxidative Medicine and Cellular Longevity·Vasilina SergeevaSvetlana Kostyuk
Sep 2, 2020·Free Radical Biology & Medicine·Jacek GrebowskiGrzegorz Litwinienko
Jun 5, 2021·Advanced Drug Delivery Reviews·Yiyuan KangLongquan Shao

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