Fully automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification and same species antibodies

Laboratory Investigation; a Journal of Technical Methods and Pathology
Wenjun ZhangLei Tang

Abstract

The ability to simultaneously visualize the presence, abundance, location and functional state of many targets in cells and tissues has been described as a true next-generation approach in immunohistochemistry (IHC). A typical requirement for multiplex IHC (mIHC) is the use of different animal species for each primary (1°Ab) and secondary (2°Ab) antibody pair. Although 1°Abs from different species have been used with differently labeled species-specific 2°Abs, quite often the appropriate combination of antibodies is not available. More recently, sequential detection of multiple antigens using 1°Abs from the same species used a microwaving treatment between successive antigen detection cycles to elute previously bound 1°Ab/2°Ab complex and therefore to prevent the cross-reactivity of anti-species 2°Abs used in subsequent detection cycles. We present here a fully automated 1°Ab/2°Ab complex heat deactivation (HD) method on Ventana's BenchMark ULTRA slide stainer. This method is applied to detection using fluorophore-conjugated tyramide deposited on the tissue and takes advantage of the strong covalent bonding of the detection substrate to the tissue, preventing its elution in the HD process. The HD process was characterized for (...Continue Reading

References

Feb 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G T Campbell, A S Bhatnagar
Dec 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Kolodziejczyk, A J Baertschi
Jan 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Y LanR C Atkins
Feb 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G L FerriG Giro
Feb 19, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·A MoreauC Laboisse
Feb 19, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·T Le NeelA Truchaud
Jun 14, 2005·Acta histochemica·Igor B BuchwalowWerner Boecker
Jan 24, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Zsuzsanna E Tóth, Eva Mezey
Feb 19, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Daniel PiriciRadu Stanescu
Mar 18, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ningshan WangRoy Freeman
Apr 14, 2011·MAbs·Josef Vlasak, Roxana Ionescu
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Michael J GerdesFiona Ginty
Mar 4, 2014·Nature Medicine·Michael AngeloGarry P Nolan
Apr 1, 2014·Nature Methods·David L Rimm
May 6, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rossella GendusaGiorgio Cattoretti
Nov 27, 2014·Archives of Pathology & Laboratory Medicine·Jeffrey W Prichard
Nov 22, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·William A DayLarry E Morrison

❮ Previous
Next ❯

Citations

Feb 16, 2019·Transplantation·Michelle A Wood-TrageserAnthony J Demetris
Mar 18, 2020·Expert Review of Molecular Diagnostics·Vesal YaghoobiMalini Harigopal
Apr 18, 2020·Cancer Communications·Wei Chang Colin TanTony Kiat Hon Lim
Nov 13, 2019·Archives of Pathology & Laboratory Medicine·Eric E WalkUNKNOWN College of American Pathologists Personalized Health Care Committee
Apr 29, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Larry E MorrisonDaniel R Bauer
Oct 14, 2017·Analytical and Bioanalytical Chemistry·Aldo RodaPatrizia Simoni
Apr 30, 2019·The Journal of Clinical Investigation·Jeeba KuriakoseHans Häcker
Jan 9, 2019·Frontiers in Oncology·Lars Ole SchwenOliver Grimm
Dec 20, 2019·Nucleic Acids Research·Lena Voith von VoithenbergGovind V Kaigala
Jul 23, 2020·Cell Reports·Sajjan KoiralaPatrick Ryan Potts
Dec 4, 2020·Journal for Immunotherapy of Cancer·Siwen Hu-LieskovanHolden T Maecker
Apr 4, 2020·Journal of Neuroscience Methods·Alexander J EhrenbergLea T Grinberg
May 1, 2021·Cells·Markus TiemannIgor Buchwalow
Mar 27, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael A CannarileVaios Karanikas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved