Fully-automated, chemiluminescence IgA and IgG anti-tissue transglutaminase (tTG) antibodies serum assays for the screening of celiac disease

Journal of Immunological Methods
Massimo DavesGiuseppe Lippi

Abstract

Celiac disease (CD) is a systemic immune-mediated enteropathy sustained by gluten ingestion in genetically susceptible subjects. The European Society for Pediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) has recently revised the diagnostic criteria, emphasizing the crucial role of serological testing in the diagnosis of this condition. This study was hence aimed to evaluate a new chemiluminescence assay for measuring anti-transglutaminase (tTG) and anti-endomysium (EMA) antibodies in a general population of unselected outpatients. The IgA and IgG anti-tissue transglutaminase (tTG) antibodies (Quanta Flash® IgA and Quanta Flash® IgG tTG, Inova Diagnostics, San Diego, CA, USA) were measured with the fully-automated BIO-FLASH® analyzer (Inova Diagnostics) in serum samples of 727 consecutive patients without a diagnosis of CD. Data were compared with those of anti-endomysium antibody (EmA) obtained in the same population. A total of 96.4% samples display a negative concordance (anti-tTG negative and EMA negative), O% were positive for EMA and negative for anti-tTG IgA and IgG, 3.6% were both positive for tTG IgA and EMA, whereas 0.6% displayed discordant results (positive for anti-tTG and negative for EMA). The concord...Continue Reading

References

Dec 31, 2004·Journal of Pediatric Gastroenterology and Nutrition·Ivor D HillUNKNOWN North American Society for Pediatric Gastroenterology, Hepatology and Nutrition
Sep 8, 2012·Gastroenterology Research and Practice·Govind K MakhariaC J J Mulder
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Jan 21, 2014·Autoimmunity Reviews·Elio Tonutti, Nicola Bizzaro

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Citations

Oct 11, 2017·Journal of Pediatric Gastroenterology and Nutrition·Giulia PrevitaliMaria G Alessio
Nov 24, 2017·Journal of Clinical Laboratory Analysis·Johannes WolfThomas Mothes
Apr 19, 2020·Journal of Pediatric Gastroenterology and Nutrition·Michelle Hjelm, Ala K Shaikhkhalil

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