Fully automated processing of buffy-coat-derived pooled platelet concentrates

Transfusion
Karin JanetzkoHermann Eichler

Abstract

The OrbiSac device, which was developed to automate the manufacture of buffy-coat PLT concentrates (BC-PCs), was evaluated. In-vitro characteristics of BC-PC preparations using the OrbiSac device were compared with manually prepared BC-PCs. For standard processing (Std-PC, n = 20), four BC-PCs were pooled using 300 mL of PLT AS (PAS) followed by soft-spin centrifugation and WBC filtration. The OrbiSac preparation (OS-PC, n = 20) was performed by automated pooling of four BC-PCs with 300 mL PAS followed by centrifugation and inline WBC filtration. All PCs were stored at 22 degrees C. Samples were withdrawn on Day 1, 5, and 7 evaluating PTL count, blood gas analysis, glucose, lactate, LDH, beta-thromboglobulin, hypotonic shock response, and CD62p expression. A PLT content of 3.1 +/- 0.4 x 10(11) (OS-PCs) versus 2.7 +/- 0.5 x 10(11) (Std-PCs, p < 0.05) was found. A CV of 19 percent (Std-PC) versus 14 percent (OS-PC) suggests more standardization in the OS group. At Day 7, the Std-PCs versus OS-PCs showed a glucose consumption of 1.03 +/- 0.32 micro mol per 10(9) PLT versus 0.75 +/- 0.25 micro mol per 10(9) PLT (p < 0.001), and a lactate production of 1.50 +/- 0.86 micro mol per 10(9) versus 1.11 +/- 0.61 micro mol per 10(9) (p < 0...Continue Reading

References

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Jul 26, 2003·Transfusion Medicine Reviews·Alan T Tinmouth, John Freedman

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Citations

Apr 26, 2011·International Journal of Hematology·Xiao-Yan DengChuan-Xi Wang
Apr 12, 2005·Transfusion·Scott Murphy
Sep 5, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Lisa Denesiuk, Gwen Clarke
Sep 21, 2011·Cytotherapy·Vijayendran GovindasamyRamesh R Bhonde
Apr 9, 2011·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·S Clément
Apr 26, 2011·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Pieter F van der Meer, Dirk de Korte
May 19, 2009·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·R TardivelH Isola
Jun 16, 2007·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Joan CidRamon Pau Pla
Apr 12, 2005·Transfusion·Luis LarreaMaría Angeles Soler
Mar 30, 2016·Progress in Polymer Science·Fa-Ming Chen, Xiaohua Liu

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