Fully automated single-use stirred-tank bioreactors for parallel microbial cultivations

Bioprocess and Biosystems Engineering
Andreas KustererDirk Weuster-Botz

Abstract

Single-use stirred tank bioreactors on a 10-mL scale operated in a magnetic-inductive bioreaction block for 48 bioreactors were equipped with individual stirrer-speed tracing, as well as individual DO- and pH-monitoring and control. A Hall-effect sensor system was integrated into the bioreaction block to measure individually the changes in magnetic field density caused by the rotating permanent magnets. A restart of the magnetic inductive drive was initiated automatically each time a Hall-effect sensor indicates one non-rotating gas-inducing stirrer. Individual DO and pH were monitored online by measuring the fluorescence decay time of two chemical sensors immobilized at the bottom of each single-use bioreactor. Parallel DO measurements were shown to be very reliable and independently from the fermentation media applied in this study for the cultivation of Escherichia coli and Saccharomyces cerevisiae. The standard deviation of parallel pH measurements was pH 0.1 at pH 7.0 at the minimum and increased to a standard deviation of pH 0.2 at pH 6.0 or at pH 8.5 with the complex medium applied for fermentations with S. cerevisiae. Parallel pH-control was thus shown to be meaningful with a tolerance band around the pH set-point of +/...Continue Reading

References

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Citations

Jul 14, 2010·Applied Microbiology and Biotechnology·Torben HoefelDirk Weuster-Botz
Dec 25, 2010·Applied Microbiology and Biotechnology·Ralf Hortsch, Dirk Weuster-Botz
Mar 21, 2008·Bioprocess and Biosystems Engineering·Dirk Weuster-Botz
Oct 17, 2013·Microbial Cell Factories·Patrick SagmeisterChristoph Herwig
Feb 27, 2010·Current Opinion in Biotechnology·Peter Neubauer, Stefan Junne
Dec 4, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hendrik KortmannAndreas Schmid
Apr 28, 2011·Biotechnology Progress·Gabi GebhardtDirk Weuster-Botz
Jan 4, 2012·Biotechnology Journal·Michael Schmidt, Dirk Weuster-Botz
Jul 30, 2010·Biotechnology Journal·Evgenij LyaginMatthias Kraume
Nov 27, 2009·Biotechnology Progress·Ralf Hortsch, Dirk Weuster-Botz
Mar 4, 2010·Biotechnology and Bioengineering·Ralf HortschDirk Weuster-Botz
Jun 28, 2015·Journal of Biotechnology·Andreas SchmidederDirk Weuster-Botz
May 15, 2015·Bioprocess and Biosystems Engineering·Nils H JanzenDirk Weuster-Botz
Jun 17, 2014·Metabolic Engineering·Stéphanie HeuxJean-Charles Portais
Jun 25, 2017·Journal of Biotechnology·Benjamin KickDirk Weuster-Botz
Aug 23, 2019·Microbial Ecology·Yihua SunMeng Li
Mar 3, 2017·Journal of Industrial Microbiology & Biotechnology·Andreas Schmideder, Dirk Weuster-Botz
Aug 1, 2017·Engineering in Life Sciences·Sarah Theresa PoschenriederKathrin Castiglione
Nov 14, 2016·Engineering in Life Sciences·David Benjamin NickelAndreas Knepper

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