Fully Human Immunoglobulin G From Transchromosomic Bovines Treats Nonhuman Primates Infected With Ebola Virus Makona Isolate.

The Journal of Infectious Diseases
Thomas LukePeter B Jahrling

Abstract

Transchromosomic bovines (Tc-bovines) adaptively produce fully human polyclonal immunoglobulin (Ig)G antibodies after exposure to immunogenic antigen(s). The National Interagency Confederation for Biological Research and collaborators rapidly produced and then evaluated anti-Ebola virus IgG immunoglobulins (collectively termed SAB-139) purified from Tc-bovine plasma after sequential hyperimmunization with an Ebola virus Makona isolate glycoprotein nanoparticle vaccine. SAB-139 was characterized by several in vitro production, research, and clinical level assays using wild-type Makona-C05 or recombinant virus/antigens from different Ebola virus variants. SAB-139 potently activates natural killer cells, monocytes, and peripheral blood mononuclear cells and has high-binding avidity demonstrated by surface plasmon resonance. SAB-139 has similar concentrations of galactose-α-1,3-galactose carbohydrates compared with human-derived intravenous Ig, and the IgG1 subclass antibody is predominant. All rhesus macaques infected with Ebola virus/H.sapiens-tc/GIN/2014/Makona-C05 and treated with sufficient SAB-139 at 1 day (n = 6) or 3 days (n = 6) postinfection survived versus 0% of controls. This study demonstrates that Tc-bovines can produ...Continue Reading

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Citations

Nov 27, 2018·The Journal of Infectious Diseases·Robert W CrossJohn H Connor
Apr 27, 2021·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Deepak KumarNagendra R Hegde

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Methods Mentioned

BETA
Enzyme-linked immunosorbent assay
flow cytometry
ELISA
Enzyme-Linked
ELISAs
surface plasmon resonance
sedation
chip
glycosylation

Clinical Trials Mentioned

NCT02788188
NCT02508584

Software Mentioned

R
JMP
FlowJo
JMP Pro
Spotfire S +
Gene
Tibco

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