Fully integrated miniature device for automated gene expression DNA microarray processing

Analytical Chemistry
Robin H LiuAndy McShea

Abstract

A DNA microarray with 12,000 features was integrated with a microfluidic cartridge to automate the fluidic handling steps required to carry out a gene expression study of the human leukemia cell line (K562). The fully integrated microfluidic device consists of microfluidic pumps/mixers, fluid channels, reagent chambers, and a DNA microarray silicon chip. Microarray hybridization and subsequent fluidic handling and reactions (including a number of washing and labeling steps) were performed in this fully automated and miniature device before fluorescent image scanning of the microarray chip. Electrochemical micropumps were integrated into the cartridge to provide pumping of liquid solutions. The device was completely self-contained: no external pressure sources, fluid storage, mechanical pumps, mixers, or valves were necessary for fluid manipulation, thus eliminating possible sample contamination and simplifying device operation. Fluidic experiments were performed to study the on-chip washing efficiency and uniformity. A single-color transcriptional analysis of K562 cells with a series of calibration controls (spiked-in controls) to characterize this new platform with regard to sensitivity, specificity, and dynamic range was perf...Continue Reading

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Citations

Sep 12, 2008·Applied Microbiology and Biotechnology·Daniel PiochBritta Jürgen
Oct 10, 2007·Veterinary Research Communications·P Ajmone MarsanR Mazza
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Aug 20, 2019·Life Sciences in Space Research·Kianoosh PeyvanAndrew Pohorille
Aug 7, 2009·Journal of the American Chemical Society·Somenath RoyZhiqiang Gao
Jan 18, 2013·Analytical Chemistry·Zhiqiang GaoYuqian Ren

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