Fumarate hydratase isoforms of Leishmania major: subcellular localization, structural and kinetic properties

International Journal of Biological Macromolecules
Patrícia R FelicianoM Cristina Nonato

Abstract

Fumarate hydratases (FHs; EC 4.2.1.2) are enzymes that catalyze the reversible hydration of fumarate to S-malate. Parasitic protists that belong to the genus Leishmania and are responsible for a complex of vector-borne diseases named leishmaniases possess two genes that encode distinct putative FH enzymes. Genome sequence analysis of Leishmania major Friedlin reveals the existence of genes LmjF24.0320 and LmjF29.1960 encoding the putative enzymes LmFH-1 and LmFH-2, respectively. In the present work, the FH activity of both L. major enzymes has been confirmed. Circular dichroism studies suggest important differences in terms of secondary structure content when comparing LmFH isoforms and even larger differences when comparing them to the homologous human enzyme. CD melting experiments revealed that both LmFH isoforms are thermolabile enzymes. The catalytic efficiency under aerobic and anaerobic environments suggests that they are both highly sensitive to oxidation and damaged by oxygen. Intracellular localization studies located LmFH-1 in the mitochondrion, whereas LmFH-2 was found predominantly in the cytosol with possibly also some in glycosomes. The high degree of sequence conservation in different Leishmania species, togethe...Continue Reading

References

Apr 28, 1988·Biochimica Et Biophysica Acta·S A WoodsJ R Guest
Oct 1, 1986·Bioscience Reports·B T Kinsella, S Doonan
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jun 1, 1994·The Journal of Clinical Investigation·T BourgeronP Rustin
Jun 1, 1994·Archives of Biochemistry and Biophysics·D H Flint
Jul 2, 1998·Archives of Biochemistry and Biophysics·T MizobataY Kawata
Oct 8, 1999·Lancet·B L Herwaldt
Jul 11, 2002·Molecular and Biochemical Parasitology·Eizo TakashimaKiyoshi Kita
Jul 26, 2002·The Journal of Biological Chemistry·Sébastien BesteiroFrédéric Bringaud
Jul 19, 2003·European Journal of Biochemistry·Véronique HannaertPaul A M Michels
Dec 8, 2004·The Journal of Biological Chemistry·Ganga KrishnamurthyAmitabha Mukhopadhyay
Jan 14, 2005·The Journal of Biological Chemistry·Susanne W H van WeeldenAloysius G M Tielens
Apr 14, 2005·International Journal for Parasitology·Ross L Coppel, Casilda G Black
Jul 16, 2005·Science·Alasdair C IvensPeter J Myler
Nov 1, 2005·Lancet·Henry W MurrayNancy G Saravia
Mar 21, 2006·Molecular and Biochemical Parasitology·Fred R Opperdoes, Jean-Pierre Szikora
Apr 8, 2006·Protein Expression and Purification·Patrícia R FelicianoM Cristina Nonato
Jun 6, 2006·Molecular and Biochemical Parasitology·Alejandro LerouxCristina Nowicki
Jul 22, 2006·The Journal of Biological Chemistry·Virginie CoustouFrédéric Bringaud
Oct 7, 2006·Biochimica Et Biophysica Acta·Paul A M MichelsVéronique Hannaert
Feb 27, 2007·FEMS Microbiology Letters·Takefumi ShimoyamaKazuya Watanabe
Jun 19, 2007·Nature Genetics·Christopher S PeacockMatthew Berriman
May 15, 2008·BMC Structural Biology·Carolina Perez-Iratxeta, Miguel A Andrade-Navarro
Feb 10, 2009·Current Medicinal Chemistry·Lukasz KedzierskiKatherine Kedzierska
Jun 13, 2009·Microbiology·Barbara M A van Vugt-LussenburgPeter-Leon Hagedoorn

❮ Previous
Next ❯

Citations

Jul 12, 2016·Veterinary Parasitology·Rosa Ma Bermúdez-CruzM Guadalupe Ortega-Pierres
Aug 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Patricia R FelicianoM Cristina Nonato
Feb 17, 2018·The Journal of Biological Chemistry·Vijay JayaramanHemalatha Balaram
Jun 14, 2015·Applied and Environmental Microbiology·Miriam KronenIvan A Berg
Feb 15, 2019·The FEBS Journal·Mariana A Ajalla AleixoMaria Cristina Nonato
Dec 15, 2015·The Biochemical Journal·Renata Almeida Garcia ReisMaria Cristina Nonato
Jan 15, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Ricardo Augusto Pereira de Pádua, Maria Cristina Nonato
Feb 8, 2021·International Journal of Biological Macromolecules·Iara Aimê CardosoMaria Cristina Nonato
Jan 16, 2019·ACS Chemical Biology·Patricia R FelicianoMaria Cristina Nonato

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Dermatologic Therapy
Karan K SraStephen K Tyring
Indian Journal of Pathology & Microbiology
Bushra MoizNatasha Ali
Indian Journal of Pathology & Microbiology
Bushra MoizNatasha Ali
© 2021 Meta ULC. All rights reserved