FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis

EBioMedicine
Lianpin WuJiongyu Chen

Abstract

FUN14 domain-containing 1 (FUNDC1), as a novel member of mitochondria-associated endoplasmic reticulum (ER) membranes associates with mitochondrial division and mitophagy. However, the expression profile and functional roles of FUNDC1 remain largely unclear in human cancer biology, including breast cancer (BC). Immunohistochemistry and western blot analysis were used to determine the expression of FUNDC1 and BMI1 polycomb ring finger oncogene (BMI1). CCK8, cell counting and transwell assays were used to analyze cell proliferation, migration and invasion, respectively. Luciferase reporter and chromatin immunoprecipitation (ChIP) assays were used to detect the transcriptional regulation of Nuclear factor of activated T-cells, cytoplasmic 1 (NFATC1). The prognostic merit of NFATC1 expression was assessed by Kaplan-Meier assay. Immunohistochemistry revealed strong immunostaining for FUNDC1 in cytoplasmic and nuclear membrane distribution in BC tissues as compared with normal breast epithelium. Kaplan-Meier survival analysis showed worse outcome for BC patients with high FUNDC1 expression. In vitro assay of gain- and loss-of-function of FUNDC1 suggested that FUNDC1 could stimulate BC cell proliferation, migration and invasion. Furth...Continue Reading

Citations

May 28, 2019·Cells·Monica Vara-PerezPatrizia Agostinis
Jan 24, 2020·Antioxidants & Redox Signaling·Lei LiuQuan Chen
Feb 7, 2020·Cells·Ying WangZheng Dong
Jan 10, 2021·EBioMedicine·Dan YangQi-Zhu Tang
Feb 9, 2021·Journal of Drug Targeting·Yu-Han QiuRong-Guang Shao
Feb 14, 2021·Cellular and Molecular Life Sciences : CMLS·Logan P Poole, Kay F Macleod
Jan 30, 2021·Frontiers in Cell and Developmental Biology·Aurora Gil-HernándezAlejandro Silva-Palacios
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Hongjiao YuDu Feng
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Jul 19, 2021·Molecular Neurobiology·Mimi WuZhengliang Ma

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Methods Mentioned

BETA
PCR
Assay
immunoprecipitation
ChIP
Protein Assay
FACS
transfection

Software Mentioned

FlowJo
GenExMiner
ImageJ
SPSS
Kaplan Plotter
GraphPad Prism
GraphPad

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