Function and expression of CD1d and invariant natural killer T-cell receptor in the cotton rat (Sigmodon hispidus)

Immunology
Alina Suzann FichtnerThomas Herrmann

Abstract

The cotton rat (Sigmodon hispidus) belongs to the rodent family of Cricetidae and provides a powerful model to study the pathogenesis of human respiratory viruses and measles virus. Recent studies in other rodent models have suggested a role for invariant natural killer T (iNKT) cells in antiviral immunity and vaccination against respiratory virus infections. Using new experimental tools, we provide the first evidence for a functional CD1d cell molecule (crCD1d) and iNKT T-cell receptor in cotton rats. The crCD1d cDNA sequence was identified and crCD1d transductants showed that monoclonal antibody WTH-2 stains crCD1d as efficiently as mouse or rat CD1d. The expression of crCD1d was clearly weaker for thymocytes and B cells, and higher for T cells, which is different to what is found in murine species. The antigen-presenting capacity of crCD1d was demonstrated with crCD1d-immunoglobulin dimers loaded with the glycolipid PBS57, which bound iNKT T-cell receptors. Evidence for functional cotton rat iNKT cells was provided by detection of interferon-γ and interleukin-4 in cultures of splenocytes stimulated with PBS57 and α-galactosylceramide and by specific staining of about 0·2% of splenocytes with PBS57-loaded crCD1d dimers. Canon...Continue Reading

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Citations

Jan 20, 2016·Immunology
Nov 26, 2015·PloS One·Daniel PalettaThomas Herrmann
Feb 20, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guan YangJohn P Driver
Nov 17, 2020·Frontiers in Immunology·John P DriverJürgen A Richt

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