Function of a glutathione-dependent formaldehyde dehydrogenase in Rhodobacter sphaeroides formaldehyde oxidation and assimilation

Biochemistry
R D Barber, T J Donohue

Abstract

Despite its reactivity with many biological molecules, formaldehyde can be commonly encountered by virtually all cells. The widespread existence of glutathione-dependent formaldehyde dehydrogenases (GSH-FDH) in procaryotes and eucaryotes suggests this enzyme plays a central and universal role in biological formaldehyde oxidation. This work sought to determine the role of GSH-FDH in the facultative phototrophic bacterium Rhodobacter sphaeroides. Growth phenotypes of wild-type and mutant cells, changes in enzyme specific activities, and the pattern of 13C-labeled compounds detected by NMR spectroscopy cumulatively suggest that R. sphaeroides GSH-FDH can play a critical role in formaldehyde metabolism under both photosynthetic and aerobic respiratory conditions. In photosynthetic cells, the data indicate that GSH-FDH generates reducing power, in the form of NADH, and one-carbon skeletons that are oxidized to carbon dioxide for subsequent assimilation by the Calvin-Benson-Bassham cycle. For example, use of methanol as a sole photosynthetic carbon source increases the specific activities of GSH-FDH, an NAD-dependent formate dehydrogenase, and the key Calvin-Benson-Bassham cycle enzyme, ribulose-1,5-bisphosphate carboxylase. This rol...Continue Reading

References

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Citations

Jan 4, 2003·Journal of Bacteriology·Carlos Rios-VelazquezTimothy J Donohue
Nov 18, 2004·Journal of Bacteriology·Jason W HickmanTimothy J Donohue
Aug 2, 2012·BMC Microbiology·Stephen P KiddAlastair G McEwan
May 8, 2001·Archives of Biochemistry and Biophysics·C Ríos-VelázquezT J Donohue
May 13, 2014·Applied Microbiology and Biotechnology·Jan T KeltjensHuub J M Op den Camp
Apr 17, 2010·Bulletin of Environmental Contamination and Toxicology·Zhongjun Xu, Haiping Hou
Mar 15, 2016·Frontiers in Microbiology·Nathan H ChenAlastair G McEwan
Sep 2, 2005·Molecular Microbiology·Stephen P KiddAlastair G McEwan
May 7, 2015·Environmental Microbiology·Martin TaubertJ Colin Murrell
Mar 30, 2011·Environmental Microbiology·Ludmila Chistoserdova
Jul 22, 1998·Journal of Molecular Biology·R D Barber, T J Donohue

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