Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation. Regulation of energy transfer and ferredoxin-mediated NADP+ reduction on the cytoplasmic side

The Journal of Biological Chemistry
Yusuke TsukataniHirozo Oh-Oka

Abstract

The PscD subunit in the homodimeric "type I" photosynthetic reaction center (RC) complex of the green sulfur bacterium Chlorobium tepidum was disrupted by insertional mutagenesis of its relevant pscD gene. This is the first report on the use of the direct mutagenic approach into the RC-related genes in green sulfur bacteria. The RC complex of C. tepidum is supposed to form a homodimer of two identical PscA subunits together with three other subunits: PscB (FA/FB-containing protein), PscC (cytochrome cz), and PscD. PscD shows a relatively low but significant similarity in its amino acid sequence to PsaD in the photosystem I of plants and cyanobacteria. We studied the biochemical and spectroscopic properties of a mutant lacking PscD in order to elucidate its unknown function. 1) The RC complex isolated from the mutant cells showed no band corresponding to PscD on SDS-PAGE analysis. 2) The growth rate of the PscD-less mutant was slower than that of the wild-type cells at low light intensities. 3) Time-resolved fluorescence spectra at 77 K revealed prolonged decay times of the fluorescence from bacteriochlorophyll c on the antenna chlorosome and from bacteriochlorophyll a on the Fenna-Matthews-Olson antenna protein in the mutant ce...Continue Reading

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Citations

Jul 2, 2009·Photosynthesis Research·Masayuki Komura, Shigeru Itoh
Apr 21, 2010·Photosynthesis Research·Steven P Romberger, John H Golbeck
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jiro HaradaHirozo Oh-oka
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Richard KurasCatherine de Vitry
Nov 22, 2015·Photosynthesis Research·David BínaRadek Litvín
Jun 1, 2016·Microbiological Research·Beatrycze Nowicka, Jerzy Kruk
Jul 18, 2006·Photochemistry and Photobiology·Hirozo Oh-oka
Apr 5, 2020·International Journal of Molecular Sciences·Dorota KowalskaSebastian Mackowski
Aug 22, 2012·Frontiers in Microbiology·Kajetan VoglDonald A Bryant

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