Function of Brassica napus BnABI3 in Arabidopsis gs1 , an Allele of AtABI3 , in Seed Development and Stress Response

Frontiers in Plant Science
Peipei Xu, Weiming Cai

Abstract

Abscisic acid (ABA) has been implicated in plant adaptation to various environmental stresses in addition to the regulation of seed dormancy and leaf senescence. ABI3 is a B3 domain-containing family protein and functions in the ABA signaling pathway during seed development. To date, the ABI3 orthologous have not been studied in Brassica napus. The aim of this study is to investigate the function of BnABI3 in plant development and stress response. Here, we identified an Arabidopsis line (gs1) from a population of mutagenized seeds and showed that GS1 is a new allele of AtABI3. When the Arabidopsis gs1 mutant was transformed with the BnABI3 gene, the transformed plants produced seeds that turned yellow and acquired desiccation tolerance. Moreover, BnABI3 regulates seed coat development and mucilage secretion by directly targeting the AtMUM1 and AtGATL5 genes. In addition, we showed that BnABI3 expression rescued gs1 freezing-induced green seed coloration by targeting AtSGR1/2 in transgenic Arabidopsis. BnABI3 is also involved in lateral root development and conferred a novel interaction between ABA and auxin signaling in roots. The potential role of ABI3 protein in endoplasmic reticulum homoeostasis was also tested. Altogether, ...Continue Reading

References

Oct 1, 1992·The Plant Cell·J GiraudatH M Goodman
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Jan 15, 2000·The Journal of Biological Chemistry·H ChoiS Y Kim
Feb 24, 2001·Trends in Plant Science·A RohdeM Holdsworth
Dec 12, 2001·The Plant Journal : for Cell and Molecular Biology·M SuzukiD R McCarty
May 3, 2005·Annual Review of Plant Biology·Eiji Nambara, Annie Marion-Poll
Aug 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jian MaoHong-Quan Yang
May 13, 2008·Biochemical and Biophysical Research Communications·Huaqin He, Jiaxu Li
Mar 27, 2009·Planta·Lisette Maria Catharina NitschWim Hendrik Vriezen
Jan 30, 2010·Science·A KhandelwalR S Quatrano
Feb 9, 2010·Trends in Plant Science·Ruthie AngeloviciAaron Fait
Mar 31, 2011·Plant Signaling & Behavior·Doron Shkolnik-Inbar, Dudy Bar-Zvi
Apr 25, 2012·Plant Science : an International Journal of Experimental Plant Biology·Kevin N BaronClaudio Stasolla
Sep 18, 2012·Nature Cell Biology·Benjamin PéretMalcolm J Bennett
Feb 8, 2013·Annals of Botany·Thierry JoëtStéphane Dussert
Mar 15, 2013·Molecular Biology and Evolution·Barry G Hall
Mar 29, 2013·Plant Cell Reports·Kazuo Nakashima, Kazuko Yamaguchi-Shinozaki
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Frédéric DelmasMarcus A Samuel
Nov 26, 2013·The Arabidopsis Book·Ruth Finkelstein
May 23, 2015·Trends in Plant Science·Edith FrancozChristophe Dunand
Jul 7, 2015·Nature Communications·Ute VoßMalcolm J Bennett
Feb 6, 2017·Plant, Cell & Environment·Nuria Fernández-BautistaM Mar Castellano
Feb 9, 2017·Plant Science : an International Journal of Experimental Plant Biology·Peipei Xu, Weiming Cai
Nov 21, 2017·Journal of Visualized Experiments : JoVE·Benson E LindseyJelena Brkljacic
Feb 15, 2018·Plant Physiology·Dachuan ShiYingzhen Kong

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Citations

May 24, 2020·International Journal of Molecular Sciences·Hanna Kijak, Ewelina Ratajczak
May 22, 2020·Journal of Integrative Plant Biology·Ying Hua SuXian Sheng Zhang
Mar 13, 2020·Frontiers in Plant Science·Neeta LohaniPrem L Bhalla

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Methods Mentioned

BETA
Transgenic
PCR
protein assay
Assay
Immunoprecipitation
ChIP
environmental stress

Software Mentioned

GraphPad
MEGA
CLUSTALW
LightCycler 96
GraphPad Prism
DNA MAN

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