PMID: 9171423Jun 1, 1997Paper

Function of conserved histidine-243 in phosphatase activity of EnvZ, the sensor for porin osmoregulation in Escherichia coli

Journal of Bacteriology
W Hsing, T J Silhavy

Abstract

EnvZ and OmpR are the sensor and response regulator proteins of a two-component system that controls the porin regulon of Escherichia coli in response to osmolarity. Three enzymatic activities are associated with EnvZ: autokinase, OmpR kinase, and OmpR-phosphate (OmpR-P) phosphatase. Conserved histidine-243 is critical for both autokinase and OmpR kinase activities. To investigate its involvement in OmpR-P phosphatase activity, histidine-243 was mutated to several other amino acids and the phosphatase activity of mutated EnvZ was measured both in vivo and in vitro. In agreement with previous reports, we found that certain substitutions abolished the phosphatase activity of EnvZ. However, a significant level of phosphatase activity remained when histidine-243 was replaced with certain amino acids, such as tyrosine. In addition, the phosphatase activity of a previously identified kinase- phosphatase+ mutant was not abolished by the replacement of histidine-243 with asparagine. These data indicated that although conserved histidine-243 is important for the phosphatase activity, a histidine-243-P intermediate is not required. Our data are consistent with a previous model that proposes a common transition state with histidine-243 (E...Continue Reading

References

Nov 1, 1979·Journal of Bacteriology·M N Hall, T J Silhavy
Jan 1, 1992·Annual Review of Genetics·J S Parkinson, E C Kofoid
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G S LukatJ B Stock
Sep 1, 1989·Journal of Bacteriology·C M HamiltonS R Kushner
Nov 20, 1989·Journal of Molecular Biology·J M Slauch, T J Silhavy
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S ForstM Inouye
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·V Weiss, B Magasanik
Jan 1, 1988·Annual Review of Cell Biology·S Forst, M Inouye
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A J Ninfa, B Magasanik
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·A M StockJ B Stock
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·L J KenneyT J Silhavy
Aug 1, 1993·Journal of Bacteriology·J W Little
Jun 1, 1996·Molecular Microbiology·W R McCleary

❮ Previous
Next ❯

Citations

Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Sep 3, 2008·Molecular Microbiology·Albert Siryaporn, Mark Goulian
Mar 6, 2013·Antimicrobial Agents and Chemotherapy·Alina D GutuSamuel M Moskowitz
Nov 26, 1998·The Journal of Biological Chemistry·D GeorgellisE C Lin
May 16, 2000·The Journal of Biological Chemistry·M E CastelliF C Soncini
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y ZhuM Inouye
Dec 2, 2005·The EMBO Journal·Alberto MarinaWayne A Hendrickson
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Guy ShinarUri Alon
Sep 8, 2011·Molecular Microbiology·TuAnh Ngoc Huynh, Valley Stewart
Jun 19, 2009·The Journal of Biological Chemistry·Sakthimala JagadeesanPenelope I Higgs
Jan 21, 2014·Microbiology·Mario Alberto Flores-ValdezMiguel Ángel De la Cruz
Aug 4, 2011·PLoS Genetics·H Deborah ChenEduardo A Groisman
Feb 8, 2018·Nature Communications·Maite VillanuevaIñigo Lasa
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J WuA Newton
Mar 24, 2006·Molecular Microbiology·Eric Batchelor, Mark Goulian
Jul 19, 2013·Biochemical Society Transactions·Paul V Attwood
Oct 2, 2012·Journal of Bacteriology·Nikhilesh S ChandDeborah T Hung
Dec 7, 2014·Microbiology·Ryan D VanYperenJoel S Griffitts
Apr 13, 2019·International Journal of Molecular Sciences·Anjali Y BhagirathKangmin Duan
Jun 24, 2020·Journal of Bacteriology·Jérôme CoppineRégis Hallez
May 13, 2009·Molecular Microbiology·Henri GerkenRajeev Misra
Mar 13, 2010·Science·Guy Shinar, Martin Feinberg
Jul 7, 2009·Annual Review of Microbiology·Rong Gao, Ann M Stock
Jan 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eric Batchelor, Mark Goulian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.