Function of cryopreserved pig aortas

The Journal of Surgical Research
E RendalC Andión

Abstract

This paper analyzes the influence of storage in the gas phase or liquid phase on grafts, together with the thawing method (15 degrees C/min or 100 degrees C/min) on the postthawing activity of pig cryopreserved arterial grafts (aortas). Obtainment of arterial grafts (aortas) was from pigs with an ischemic time not greater than 2 h. Each aorta was divided into five fragments and assigned randomly to one control group of fresh aorta and four groups of cryopreserved aortas: group 1: gas phase/slow thawing; group 2: gas phase/rapid thawing; group 3: liquid phase/slow thawing; and group 4: liquid phase/rapid thawing. After the incubation in antibiotic solution, the cryopreservation in RPMI medium +10% DMSO was carried out and the level of cooling used was a reduction of 1 degrees C/min. The contraction and relaxation responses of the fresh and frozen/thawed arteries were carried out in organ baths. After thawing, the sensitivity to various agonists and maximal responses to the endothelium-dependent and independent relaxant agents were decreased. The maximal responses to the tested vasoconstrictors (KCl and noradrenaline) were, respectively, 13% and 24% of the responses obtained in unfrozen aortas. The endothelium-independent relaxan...Continue Reading

References

Jun 1, 1988·Trends in Pharmacological Sciences·E Müller-Schweinitzer
Jan 1, 1988·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·S L PassaniA C Newby
Oct 1, 1994·Cryobiology·C J HuntD E Pegg
Feb 1, 1994·Cryobiology·E Müller-Schweinitzer
Aug 27, 1998·Journal of Vascular Surgery·F StankeP Devillier

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Citations

May 24, 2007·Tissue Engineering·Markus HoenickaDietrich E Birnbaum
Jun 4, 2014·The Journal of Surgical Research·Tristan P C van DoormaalAlbert van der Zwan
Feb 17, 2007·The Journal of Surgical Research·Brian D StemperFrank A Pintar
Sep 1, 2005·Clinics in Laboratory Medicine·Manish J Gandhi, D Michael Strong
Jan 5, 2010·Organogenesis·Else Müller-Schweinitzer

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