Function of glutathione peroxidases in legume root nodules

Journal of Experimental Botany
M A MatamorosManuel Becana

Abstract

Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the fact that reactive oxygen species are produced at different steps of the symbiosis. The function of two Gpxs that are highly expressed in nodules of the model legume Lotus japonicus was examined. Gene expression analysis, enzymatic and nitrosylation assays, yeast cell complementation, in situ mRNA hybridization, immunoelectron microscopy, and LjGpx-green fluorescent protein (GFP) fusions were used to characterize the enzymes and to localize each transcript and isoform in nodules. The LjGpx1 and LjGpx3 genes encode thioredoxin-dependent phospholipid hydroperoxidases and are differentially regulated in response to nitric oxide (NO) and hormones. LjGpx1 and LjGpx3 are nitrosylated in vitro or in plants treated with S-nitrosoglutathione (GSNO). Consistent with the modification of the peroxidatic cysteine of LjGpx3, in vitro assays demonstrated that this modification results in enzyme inhibition. The enzymes are highly expressed in the infected zone, but the LjGpx3 mRNA is also detected in the cortex and vascular bundles. LjGpx1 is localized to the plastids and nuclei, and LjGpx3 to the cytosol and endoplasmic reticulum. Based on...Continue Reading

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Citations

Apr 20, 2016·Frontiers in Plant Science·Isabelle DamianiAlexandre Boscari
Sep 20, 2018·The New Phytologist·Andreas J MeyerNicolas Rouhier
Apr 18, 2018·BioMed Research International·Uliana Ya StambulskaVolodymyr I Lushchak
Oct 27, 2018·Frontiers in Plant Science·Manuel BecanaManuel A Matamoros
Nov 3, 2020·AIMS Microbiology·Pontsho Patricia TwalaNaser Aliye Feto
Jan 17, 2021·Journal of Experimental Botany·Manuel A Matamoros, Manuel Becana

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Methods Mentioned

BETA
reverse-transcription PCR
PCR
transfection
Affinity
affinity binding
confocal microscopy

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