Function of positive regulatory gene gal4 in the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae: evidence that the GAL81 region codes for part of the gal4 protein.

Journal of Bacteriology
K MatsumotoY Oshima

Abstract

A meiotic fine structure map of the gal4 locus was constructed, which extended over 0.44 units on the chromosome (units in percent frequency of supposed recombination). Several nonsense gal4 mutations (four UAA and two supposed UGA [gal4-62 and gal4-69]) were placed at various sites on the map. In reversion experiments with 20 independently isolated gal4 mutants, only the gal4-62 and gal4-69 alleles, which are located at the same site on the map, could revert to overcome the superrepression of gal80s-1 spontaneously with a frequency of approximately 4 x 10(-7). Secondary mutations in the revertants occurred in the region of gal4-62 or were due to unlinked suppressors. A total of 15 GAL81 mutations in 19 isolates were found to be located in the same region as gal4-62 by three-point crosses with the aid of gal4 mutants; the other four could not be analyzed. The reverted gal4 gene and GAL81 mutations were semidominant over the wild-type GAL4+ allele and fully dominant over a nonsense gal4 mutation. Four suppressors (one dominant and three recessive) effective against gal4-62 and gal4-69 were isolated. The dominant suppressor was also effective against three independent, authentic auxotrophic UGA nonsense mutations, and one of the ...Continue Reading

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J E HopperL B Rowe
Dec 30, 1975·Molecular & General Genetics : MGG·A Toh-e, S Kakimoto
Oct 1, 1971·Journal of Bacteriology·J Bassel, R Mortimer
Jul 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J A ValoneN H Giles
Feb 1, 1965·Genetical Research·D WILKIE, B K LEE

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Citations

Dec 20, 1996·The Journal of Biological Chemistry·S J RussellS A Johnston
Jan 1, 1986·CRC Critical Reviews in Biochemistry·S D HanesK A Bostian
Jan 21, 2001·The Journal of Biological Chemistry·S J Russell, S A Johnston
Mar 8, 2008·The Journal of Biological Chemistry·Chase T ArcherThomas Kodadek
Jan 6, 2017·PloS One·Martin PiskacekAndrea Knight
Jun 3, 2008·The Journal of Biological Chemistry·Chase T ArcherThomas Kodadek
Apr 1, 1984·Journal of Bacteriology·B A Citron, J E Donelson
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·J M Gancedo
Dec 1, 1985·Molecular and Cellular Biology·V B Patel, N H Giles
Feb 1, 1984·Molecular and Cellular Biology·A Laughon, R F Gesteland
Feb 1, 1984·Molecular and Cellular Biology·A LaughonR F Gesteland
Aug 1, 1984·Molecular and Cellular Biology·M Johnston, R W Davis
Jul 1, 1984·Molecular and Cellular Biology·M A Post-BeittenmillerJ E Hopper

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