PMID: 8962074Dec 10, 1996Paper

Function of RNA secondary structures in transcriptional attenuation of the Bacillus subtilis pyr operon

Proceedings of the National Academy of Sciences of the United States of America
Y LuR L Switzer

Abstract

The Bacillus subtilis pyr operon is regulated by exogenous pyrimidines by a transcriptional attenuation mechanism. Transcription in vitro from pyr DNA templates specifying attenuation regions yielded terminated and read-through transcripts of the expected lengths. Addition of the PyrR regulatory protein plus UMP led to greatly increased termination. Synthetic antisense deoxyoligonucleotides were used to probe possible secondary structures in the pyr mRNA that were proposed to play roles in controlling attenuation. Oligonucleotides predicted to disrupt terminator structures suppressed termination, whereas oligonucleotides predicted to disrupt the stem of antiterminator stem-loops strongly promoted termination at the usual termination site. Oligonucleotides that disrupt a previously unrecognized stem-loop structure, called the anti-antiterminator, the formation of which interferes with formation of the downstream antiterminator, suppressed termination. We propose that transcriptional attenuation of the pyr operon is governed by switching between alternative antiterminator versus anti-antiterminator plus terminator structures, and that PyrR acts by UMP-dependent binding to and stabilization of the anti-antiterminator.

References

Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·W Salser
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M E WinklerC Yanofsky
Jan 1, 1995·Annual Review of Biochemistry·D E Draper
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P Babitzke, C Yanofsky
Apr 20, 1995·Nature·A A AntsonP Gollnick

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Citations

Sep 6, 2002·Biochimica Et Biophysica Acta·Paul Gollnick, Paul Babitzke
Feb 14, 2003·FEMS Microbiology Letters·Valentina TosatoCarlo V Bruschi
Sep 30, 2005·Nucleic Acids Research·Thirumananseri KumarevelPenmetcha K R Kumar
Jul 3, 2007·Journal of Bacteriology·Christopher J Fields, Robert L Switzer
Dec 30, 1999·Journal of Bacteriology·C Yanofsky
Apr 9, 2001·Journal of Bacteriology·J MartinussenK Hammer
Jul 3, 2004·Journal of Bacteriology·Karen E BeenkenMark S Smeltzer
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Charles L Turnbough, Robert L Switzer
Nov 20, 2012·Journal of Theoretical Biology·Amirhossein ManzourolajdadRussell L Malmberg
Sep 6, 2007·BMC Genomics·Judith HübscherBrigitte Berger-Bächi
Oct 14, 2008·BMC Genomics·Chantal W NdeWilliam E Bentley
Jun 12, 2013·Journal of Structural Biology·Balasundaresan DhakshnamoorthyPenmetcha K R Kumar
Mar 19, 2015·Frontiers in Microbiology·Elke Van AsscheHans P Steenackers
Sep 6, 2000·Journal of Molecular Biology·D CharlierN Glansdorff
Oct 28, 2016·Microbiology and Molecular Biology Reviews : MMBR·Ruben A T MarsJan Maarten van Dijl
May 19, 2019·Annual Review of Microbiology·Paul BabitzkeTony Romeo
Jul 5, 2019·Microbiology and Molecular Biology Reviews : MMBR·Charles L Turnbough
Jun 1, 2014·Biophysics Reviews·Penmetcha K R Kumar, Hiroshi Mizuno
Aug 19, 2018·Applied Microbiology and Biotechnology·Xiaoguang FanXixian Xie

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