Function of the ERFL1a Transcription Factor in Wheat Responses to Water Deficiency

International Journal of Molecular Sciences
Tian GaoGuo-Zhang Kang

Abstract

The APETALA2/ethylene response factor (AP2/ERF) superfamily is involved in the responses of plants to biotic and abiotic stresses; however, the functions and mechanisms of some members of this family in plants are unclear. In our previous study, expression of TaERFL1a, a member of the AP2/ERF family, was remarkably induced in wheat seedlings suffering freezing stress. In this study, we show that its expression was rapidly upregulated in response to salt, cold, and water deficiency, suggesting roles in the responses to abiotic stresses. Further, transient barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) resulted in significantly reduced tolerance to 20% PEG6000-stimulated water deficiency. Subcellular localization and transcriptional activation assays separately showed that TaERFL1a was targeted to the nucleus and possessed transcriptional activation activity. Yeast two-hybrid library screening identified six interacting proteins, and of these, the interactions between TaERFL1a and TaSGT1, and TaERFL1a and TaDAD2 proteins were further confirmed by yeast co-transformation and bimolecular fluorescent complementation (BiFC). Collectively, our results suggest that TaERFL1a is a stress-responsive transcription factor, wh...Continue Reading

References

Aug 13, 2002·The Plant Cell·Kim BoutilierMichiel M van Lookeren Campagne
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Arnab MukhopadhyayAkhilesh K Tyagi
Jan 13, 2006·Plant Physiology·Toshitsugu NakanoHideaki Shinshi
Mar 18, 2010·Plant Biotechnology Journal·Ruidang QuanRongfeng Huang
Aug 27, 2011·Biochimica Et Biophysica Acta·Junya MizoiKazuko Yamaguchi-Shinozaki
Oct 18, 2011·Biochimica Et Biophysica Acta·Joachim KilianDierk Wanke
Aug 4, 2012·Journal of Experimental Botany·Moshe FeldmanAbraham Korol
Mar 15, 2013·International Journal of Molecular Sciences·Søren LindemoseKaren Skriver
Jan 8, 2014·Plant Biotechnology Journal·Wei RongZengyan Zhang
Jul 19, 2014·Science·Matthias PfeiferOdd-Arne Olsen
Jul 19, 2014·Science·UNKNOWN International Wheat Genome Sequencing Consortium (IWGSC)
Jun 26, 2015·The New Phytologist·Philippa BorrillCristobal Uauy
Mar 12, 2016·Genome Génome / Conseil National De Recherches Canada·Ashokraj ShanmugamIll-Sup Nou
Oct 14, 2016·Plant Biotechnology Journal·Jonathan J PowellKemal Kazan

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY781352
BQ789086

Methods Mentioned

BETA
PCR
two-hybrid
environmental stresses
transgenic
gene knockdown
Y2H
MDA

Software Mentioned

BLAST
MEGA
DNAMAN
BLASTX
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.