Function of the natalisin receptor in mating of the oriental fruit fly, Bactrocera dorsalis (Hendel) and testing of peptidomimetics

PloS One
Shun-Hua GuiJin-Jun Wang

Abstract

Natalisins (NTLs) are conservative neuropeptides, which are only found in arthropods and are documented to regulate reproductive behaviors in insects. In our previous study, we have confirmed that NTLs regulate the reproductive process in an important agricultural pest, Bactrocera dorsalis (Hendel). Hence, in this study, to further confirm the in vivo function of NTL receptor (NTLR) and assess the potential of NTLR as an insecticide target, RNA interference targeting NTLR mRNA was performed. We found that mating frequencies of both males and females were reduced by RNAi-mediated knockdown of the NTLR transcript, while there was no effect on mating duration. Moreover, we functionally expressed the B. dorsalis NTLR in Chinese Hamster Ovary (CHO) cells and was co-transfected with an aequorin reporter to measure ligand activities. A total of 13 biostable multi-Aib analogs were tested for agonistic and antagonistic activities. While most of these NTL analogs did not show strong activity, one analog (NLFQV[Aib]DPFF[Aib]TRamide) had moderate antagonistic activity. Taken together, we provided evidence for the important roles of NTLR in regulating mating frequencies of both male and female in this fly and also provided in vitro data on ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 5, 2002·Biopolymers·M Altstein
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Mar 26, 2008·Nature·UNKNOWN Tribolium Genome Sequencing ConsortiumGregor Bucher
Jun 14, 2008·Insect Biochemistry and Molecular Biology·Michael J AikinsYoonseong Park
Nov 6, 2008·General and Comparative Endocrinology·Suparna Taneja-BageshwarRonald J Nachman
Jan 20, 2009·Bioinformatics·Andrew M WaterhouseGeoffrey J Barton
Dec 12, 2012·Frontiers in Endocrinology·Jelle CaersLiliane Schoofs
Aug 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hongbo JiangYoung-Joon Kim
Aug 1, 2015·Insect Biochemistry and Molecular Biology·Neil Audsley, Rachel E Down

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Citations

Dec 12, 2019·Frontiers in Neuroscience·Dick R NässelHonoo Satake
Feb 27, 2021·Scientific Reports·Xia-Fei WangHui Ye

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Methods Mentioned

BETA
transfection
PCR

Software Mentioned

JalView
TMHMM
CLUSTAL X2

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