PMID: 6201191Mar 13, 1984

Function of the repeating homologous sequences in nucleic acid binding domain of ribosomal protein S1

T Suryanarayana, A R Subramanian


Ribosomal protein S1 contains in its RNA binding domain four repeating, homologous stretches of sequences. Its functionally active mutant form m1-S1 [Subramanian, A.R., & Mizushima, S. (1979) J. Biol. Chem. 254, 4309] contains only three repeating stretches. In order to assess the functional importance of this repeating sequence, we cleaved S1 at its reactive SH group on Cys-349 and isolated a fragment (S1-F4) that has lost two of the homologous stretches but retains all other essential elements. We find that ribosomes reconstituted with S1-F4 instead of S1 are functionally active in translating poly(U) and poly(A) but totally inactive in translating phage MS2 RNA. The significance of this result is discussed vis-à-vis the initiation step in translating natural mRNA, and a functional role for the tetrarepeat of S1 is suggested.


Dec 1, 1979·European Journal of Biochemistry·J O ThomasA R Subramanian
Jan 2, 1978·European Journal of Biochemistry·M A MinksA R Subramanian
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·W Szer, S Leffler
Feb 18, 1970·Biochimica Et Biophysica Acta·M Smolarsky, M Tal
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·G Stöffler, H G Wittmann
Jan 1, 1982·Annual Review of Biochemistry·H G Wittmann
May 24, 1983·Biochemistry·T Suryanarayana, A R Subramanian
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J SchnierB Wittmann-Liebold
Jun 1, 1982·Bioscience Reports·R F DoolittleR A Jue


Aug 10, 2013·Journal of the American Chemical Society·Shuntaro TakahashiYoshio Okahata
Jan 11, 1991·Nucleic Acids Research·I V BoniN V Tzareva
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·Jayati SenguptaJ Frank
Mar 14, 2007·Biochemical and Biophysical Research Communications·Young Seoub ParkGyoo Yeol Jung
Apr 16, 2020·Molecular Microbiology·Muhammad S Azam, Carin K Vanderpool

Related Concepts

M1-S1 ribosomal protein
Copper Cystinate
Alkalescens-Dispar Group
Immunodiffusion Measurement
Peptide Fragments
Ribosomal Proteins
RNA, Bacterial

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