PMID: 6201191Mar 13, 1984

Function of the repeating homologous sequences in nucleic acid binding domain of ribosomal protein S1

Biochemistry
T Suryanarayana, A R Subramanian

Abstract

Ribosomal protein S1 contains in its RNA binding domain four repeating, homologous stretches of sequences. Its functionally active mutant form m1-S1 [Subramanian, A.R., & Mizushima, S. (1979) J. Biol. Chem. 254, 4309] contains only three repeating stretches. In order to assess the functional importance of this repeating sequence, we cleaved S1 at its reactive SH group on Cys-349 and isolated a fragment (S1-F4) that has lost two of the homologous stretches but retains all other essential elements. We find that ribosomes reconstituted with S1-F4 instead of S1 are functionally active in translating poly(U) and poly(A) but totally inactive in translating phage MS2 RNA. The significance of this result is discussed vis-à-vis the initiation step in translating natural mRNA, and a functional role for the tetrarepeat of S1 is suggested.

References

Dec 1, 1979·European Journal of Biochemistry·J O ThomasA R Subramanian
Jan 2, 1978·European Journal of Biochemistry·M A MinksA R Subramanian
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·W Szer, S Leffler
Feb 18, 1970·Biochimica Et Biophysica Acta·M Smolarsky, M Tal
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·G Stöffler, H G Wittmann
Jan 1, 1982·Annual Review of Biochemistry·H G Wittmann
May 24, 1983·Biochemistry·T Suryanarayana, A R Subramanian
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J SchnierB Wittmann-Liebold
Jun 1, 1982·Bioscience Reports·R F DoolittleR A Jue

Citations

Aug 10, 2013·Journal of the American Chemical Society·Shuntaro TakahashiYoshio Okahata
Jan 11, 1991·Nucleic Acids Research·I V BoniN V Tzareva
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·Jayati SenguptaJ Frank
Mar 14, 2007·Biochemical and Biophysical Research Communications·Young Seoub ParkGyoo Yeol Jung
Apr 16, 2020·Molecular Microbiology·Muhammad S Azam, Carin K Vanderpool

Related Concepts

M1-S1 ribosomal protein
Copper Cystinate
Alkalescens-Dispar Group
Immunodiffusion Measurement
Peptide Fragments
Ribosomal Proteins
RNA, Bacterial

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.