Function of untranslated regions in the mouse spermatogenesis-specific gene Tcp10 evaluated in transgenic mice.

DNA and Cell Biology
U K Ewulonu, J C Schimenti

Abstract

The mouse Tcp10 genes are transcribed exclusively in male germ cells and display multiple 5' and 3' untranslated variations generated by alternative splicing and polyadenylation signal usage. To investigate the possible role of untranslated sequences in the regulation of these genes, chimeric expression constructs with or without endogenous 5' and 3' untranslated sequences were generated and used to make transgenic mice. Analysis of these animals showed that the untranslated sequences have no effect on the transcription or translation of an attached lacZ reporter gene, thereby implying these sequences are dispensible. However, the endogenous pattern of polyadenylation site usage was altered when Tcp10 3' untranslated sequences were linked to lacZ, indicating that internal coding sequence can influence recognition of polyadenylation signals in testis. The characteristics of alternative splicing and polyadenylation signal variability reflects a common theme of promiscuity in testicular gene expression.

References

Jun 1, 1992·Molecular and Cellular Biology·J R MurtiJ C Schimenti
Jul 1, 1992·Genes & Development·F J SallésS Strickland
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N L ShaperJ H Shaper
Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D C Bullard, J C Schimenti
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S LevyO Meyuhas
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J J PeschonR D Palmiter
Mar 10, 1988·Nature·J D MarthR M Perlmutter
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·V KruysG Huez
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N Aziz, H N Munro
Jan 1, 1985·Annual Review of Genetics·L M Silver
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Apr 30, 1993·Biochemical and Biophysical Research Communications·T SuzukiK Igarashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.