Function, oligomerization and N-linked glycosylation of the Helicoverpa armigera single nucleopolyhedrovirus envelope fusion protein

The Journal of General Virology
Gang LongZhihong Hu

Abstract

In the family Baculoviridae, two distinct envelope fusion proteins are identified in budded virions (BVs). GP64 is the major envelope fusion protein of group I nucleopolyhedrovirus (NPV) BVs. An unrelated type of envelope fusion protein, named F, is encoded by group II NPVs. The genome of Helicoverpa armigera (Hear) NPV, a group II NPV of the single nucleocapsid or S type, also encodes an F-like protein: open reading frame 133 (Ha133). It was demonstrated by N-terminal sequencing of the major 59 kDa protein present in HearNPV BV that this protein is one of the two F subunits: F1 (transmembrane subunit of 59 kDa) and F2 (surface subunit of 20 kDa), both the result of cleavage by a proprotein convertase and disulfide-linked. The HearNPV F protein proved to be a functional analogue of GP64, as the infectivity of an AcMNPV gp64-deletion mutant was rescued by the introduction of the HearNPV F gene. It was also demonstrated by chemical cross-linking that HearNPV F is present in BVs as an oligomer whereby, unlike GP64, disulfide bonds are not involved. Deglycosylation assays indicated that both F1 and F2 possess N-linked glycans. However, when F was made in Hz2E5 cells, these glycans did not have an alpha-1-3 core fucose modification ...Continue Reading

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Citations

Aug 4, 2011·Journal of Invertebrate Pathology·Monique M van Oers
Sep 24, 2014·The Journal of General Virology·Monique M van OersJust M Vlak
Jan 25, 2007·Journal of Biochemistry and Molecular Biology·Dun Wang, Chuan-Xi Zhang
Sep 26, 2006·Advances in Virus Research·Monique M van Oers
Jun 13, 2009·Molekuliarnaia genetika, mikrobiologiia i virusologiia·I M Kikhno
Jan 17, 2008·The Journal of General Virology·Marcel Westenberg, Just M Vlak
Apr 30, 2017·The Journal of General Virology·Manli WangJust M Vlak

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