Functional activities of antibodies against brain-associated T cell antigens. I. Induction of T cell proliferation

European Journal of Immunology
B Jones, C A Janeway

Abstract

Rabbit anti-mouse brain antiserum mediated an in vitro cooperative interaction between Ig-anti-Ig column-purified Lyt-1+, Lyt-2- T cells and spleen B cells. DNA synthesis in the B cells was inhibited with mitomycin C, and the T cell proliferative response was measured by [3H]thymidine incorporation after 24 h of culture. The T-B cell interaction was genetically unrestricted, and the accessory function of the B cell was engaged via interaction with the Fc portion of the T cell-bound anti-mouse brain antibody since the F(ab')2 fragment was inactive. The rabbit anti-mouse brain anti-serum appears to recognize a unique antigenic determinant in the T cell membrane which triggers proliferation. Although brain-absorbed, rabbit anti-mouse thymocyte antiserum contained antibodies which bound to the T cell surface, it was incapable of inducing T cell proliferation. Furthermore, the Thy-1 molecule itself did not appear to be involved because allo- and xenoantisera against the allelic determinants were inactive, and the capping of Thy-1.2 from the T cell surface did not prevent the binding of the mitogenic component in rabbit anti-mouse brain antiserum. The T cell reaction to anti-brain antiserum is unlike the antigen-specific T cell respo...Continue Reading

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Citations

Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D P GoldC Terhorst
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Sep 1, 1984·Annales d'immunologie·M A Norcross

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