PMID: 9546240Apr 18, 1998Paper

Functional activity and transmembrane signaling mechanisms after cryopreservation of human internal mammary arteries

Journal of Vascular Surgery
E Müller-SchweinitzerW E Haefeli

Abstract

Cryopreserved human blood vessels are important tools in bypass surgery. However, several in vitro studies have demonstrated diminished postthaw functional activity. Therefore the aim of this study was to investigate the consequences of various freezing/thawing protocols and the role of protein kinase C in the postthaw functional activity of cryopreserved human arteries. In vitro responses of frozen/thawed human internal mammary arteries (IMA) were used to investigate the functional activity after thawing at 15 degrees, 30 degrees, and 100 degrees C/min and after different prefreezing equilibration times (10, 60, 120, 240 minutes) with the cryomedium (Krebs-Henseleit solution containing 1.8 mol/L dimethyl sulfoxide and 0.1 mol/L sucrose) at room temperature followed by cryostorage at -196 degrees C. Prefreezing equilibration for 10 to 120 minutes diminished maximal alpha-adrenoceptor-mediated responses to noradrenaline to approximately 60%, and equilibration for 240 minutes attenuated noradrenaline effects to less than 25% of that produced by unfrozen controls. Contractile responses were slightly better when thawing was performed at 15 degrees C/min compared with 100 degrees C/min. The postthaw sensitivity to direct activation ...Continue Reading

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Citations

May 24, 2000·British Journal of Pharmacology·E Müller-SchweinitzerW E Haefeli
Feb 5, 2010·Experimental Diabetes Research·Lora J KasselmanSeward B Rutkove
Mar 13, 2016·Journal of Immunological Methods·Antonina KlippertChristiane Stahl-Hennig
Jan 3, 2001·The Journal of Thoracic and Cardiovascular Surgery·H YokomuroT M Yau
Jan 24, 2006·The Journal of Surgical Research·Peitao WangXing Li Wang
Jul 6, 2004·The Journal of Surgical Research·E RendalC Andión
Jul 22, 2004·Cryobiology·M Esther Rendal VázquezC Andión Núñez
Feb 19, 2005·Diabetic Medicine : a Journal of the British Diabetic Association·S B RutkoveJ E Larrabee
Jan 5, 2010·Organogenesis·Else Müller-Schweinitzer

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