Functional Alleles of Chicken BG Genes, Members of the Butyrophilin Gene Family, in Peripheral T Cells

Frontiers in Immunology
Lei ChenJim Kaufman

Abstract

γδ T cells recognize a wide variety of ligands in mammals, among them members of the butyrophilin (BTN) family. Nothing is known about γδ T cell ligands in chickens, despite there being many such cells in blood and lymphoid tissues, as well as in mucosal surfaces. The major histocompatibility complex (MHC) of chickens was discovered because of polymorphic BG genes, part of the BTN family. All but two BG genes are located in the BG region, oriented head-to-tail so that unequal crossing-over has led to copy number variation (CNV) as well as hybrid (chimeric) genes, making it difficult to identify true alleles. One approach is to examine BG genes expressed in particular cell types, which likely have the same functions in different BG haplotypes and thus can be considered "functional alleles." We cloned nearly full-length BG transcripts from peripheral T cells of four haplotypes (B2, B15, B19, and B21), and compared them to the BG genes of the B12 haplotype that previously were studied in detail. A dominant BG gene was found in each haplotype, but with significant levels of subdominant transcripts in three haplotypes (B2, B15, and B19). For three haplotypes (B15, B19, and B21), most sequences are closely-related to BG8, BG9, and BG...Continue Reading

References

Apr 1, 1992·Current Opinion in Genetics & Development·T Alber
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M M MillerC G Miyada
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J SalomonsenJ Kaufman
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J KaufmanD Thorpe
Jul 1, 1988·The Journal of Heredity·K KlineB G Sanders
May 1, 1985·Tissue Antigens·K SkjødtM Simonsen
Jan 1, 1982·Immunogenetics·M SimonsenK Hála
Jan 1, 1980·Immunogenetics·M SimonsenE M Nicolaisen
Jun 4, 1999·Immunology Today·J HenryP Pontarotti
Nov 22, 1952·Nature·F H C CRICK
Oct 6, 1961·Science·L W SCHIERMAN, A W NORDSKOG
Apr 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Iain ShawJim Kaufman
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ronald M GotoMarcia M Miller
Oct 28, 2011·Trends in Immunology·Lucie Abeler-DörnerAnna Bas
Jul 26, 2014·Nature Reviews. Immunology·Heather A Arnett, Joanne L Viney
Jan 17, 2016·Annual Review of Immunology·David A RhodesJohn Trowsdale
Sep 16, 2016·Open Biology·John ChattawayJim Kaufman
Jan 20, 2017·Sub-cellular Biochemistry·Sarah E Hitchcock-DeGregori, Bipasha Barua
Jan 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Pierre VantouroutAdrian C Hayday

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Datasets Mentioned

BETA
MH156616
MH156617
MH156618
MH156619
MH156620
MH156621
MH156623
MH156624
MH156615
MH156643

Methods Mentioned

BETA
fluorescence-activated cell sorting
PCR
electrophoresis
FACS
PCRs
RNAseq
flow cytometry

Software Mentioned

Model
Powerpoint
MEGA7
DrawCoil10
BioEdit Sequence Alignment Editor
Swiss
Clustal W
CLC DNA Workbench
PyMOL
Word

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