Abstract
We previously showed that the Wnt-signaling antagonist SFRP1 (secreted frizzled-related protein 1) is a promising marker in bladder cancer. The aim of this study was to validate the prognostic role and analyze the functional significance of SFRP1. Four bladder cancer cell lines (RT112, RT4, J82 and BFTC905) and one urothelial cell line (UROtsa) were used for functional characterization of SFRP1 expression. Effects on viability, proliferation and wound healing were investigated, and canonical Wnt-pathway activity as well as Wnt-signaling target gene expression was analyzed. Additionally, tissue micro-arrays from two different bladder tumor cohorts were evaluated for SFRP1 expression, and associations with survival and histopathological parameters were analyzed. The cell lines RT112, RT4, J82 and UROtsa showed SFRP1 expression. In BFTC905, SFRP1 expression was inhibited by promoter hypermethylation. Wnt-pathway activity was absent in all cell lines and independent from SFRP1 expression. RT112 and BFTC905 were used for further functional characterization. SFRP1 overexpression resulted in decreased viability and migration in BFTC905 cells. Knockdown of SFRP1 expression in RT112 cells resulted only in marginal effects. In bladder tu...Continue Reading
References
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T E CareyL J Old
Sep 1, 1995·British Journal of Cancer·K M RiegerI C Summerhayes
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·P W FinchJ S Rubin
Sep 21, 2001·Environmental Health Perspectives·M R RossiD A Sens
Oct 11, 2001·Oncogene·F UgoliniM J Pébusque
May 9, 2003·Development·Taijiro YabeMasahiko Hibi
Jun 12, 2003·British Journal of Cancer·I ThievessenW A Schulz
Sep 12, 2003·Developmental Cell·Michael T VeemanRandall T Moon
Feb 18, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Robert StoehrArndt Hartmann
Oct 9, 2004·Cancer Science·Toshio TakadaToshikazu Ushijima
May 19, 2005·Cancer Research·Dimitri LodyginHeiko Hermeking
Jun 9, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jan LehmannUNKNOWN AUO-AB 05/95
Aug 20, 2005·Nature Reviews. Cancer·Xue-Ru Wu
Dec 15, 2005·Carcinogenesis·Margaret A Knowles
Feb 2, 2006·Oncogene·J VeeckE Dahl
Mar 14, 2007·Oncogene·E DahlC G Hammerschmied
Apr 20, 2007·International Journal of Cancer. Journal International Du Cancer·Yu-Lueng ShihYa-Wen Lin
Apr 10, 2008·Der Urologe. Ausg. A·J HatinaW A Schulz
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Dec 5, 2008·Scandinavian Journal of Urology and Nephrology. Supplementum·Margaret A Knowles
Feb 2, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J FerlayE Steliarova-Foucher
Jan 25, 2011·European Urology·Andrew H FeiferHarry W Herr
Sep 10, 2011·Acta Biochimica Et Biophysica Sinica·Antara De
Citations
Jul 8, 2016·Oncotarget·Wenlong ChengYong-Jun Chen
Aug 27, 2016·Oncotarget·Neveen Said
Feb 24, 2017·Cell Death & Disease·Wenlong ChengYongjun Chen
Jan 10, 2019·BMC Genomics·Robert AltwasserImad Shams
Dec 20, 2020·Cellular Signalling·Guanlin WuGang Fan