PMID: 9190809Jun 1, 1997Paper

Functional analyses of a variety of chimeric dioxygenases constructed from two biphenyl dioxygenases that are similar structurally but different functionally

Journal of Bacteriology
N KimuraK Furukawa

Abstract

The biphenyl dioxygenases (BP Dox) of strains Pseudomonas pseudoalcaligenes KF707 and Pseudomonas cepacia LB400 exhibit a distinct difference in substrate ranges of polychlorinated biphenyls (PCB) despite nearly identical amino acid sequences. The range of congeners oxidized by LB400 BP Dox is much wider than that oxidized by KF707 BP Dox. The PCB degradation abilities of these BP Dox were highly dependent on the recognition of the chlorinated rings and the sites of oxygen activation. The KF707 BP Dox recognized primarily the 4'-chlorinated ring (97%) of 2,5,4'-trichlorobiphenyl and introduced molecular oxygen at the 2',3' position. The LB400 BP Dox recognized primarily the 2,5-dichlorinated ring (95%) of the same compound and introduced O2 at the 3,4 position. It was confirmed that the BphA1 subunit (iron-sulfur protein of terminal dioxygenase encoded by bphA1) plays a crucial role in determining the substrate selectivity. We constructed a variety of chimeric bphA1 genes by exchanging four common restriction fragments between the KF707 bphA1 and the LB400 bphA1. Observation of Escherichia coli cells expressing various chimeric BP Dox revealed that a relatively small number of amino acids in the carboxy-terminal half (among 20 ...Continue Reading

References

Aug 1, 1979·Applied and Environmental Microbiology·K FurukawaA Kamibayashi
Jan 1, 1992·Annual Review of Microbiology·J R Mason, R Cammack
Dec 1, 1992·Microbiological Reviews·J R van der MeerA J Zehnder
May 1, 1987·Applied and Environmental Microbiology·D L BedardM J Brennan
Apr 1, 1994·Journal of Bacteriology·K FurukawaK Nakamura
Nov 1, 1993·Applied and Environmental Microbiology·B D Erickson, F J Mondello
Jan 1, 1996·Bioscience, Biotechnology, and Biochemistry·N KimuraK Furukawa

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Citations

May 29, 2016·Environmental Science and Pollution Research International·Jaya Chakraborty, Surajit Das
Mar 10, 2016·Journal of Bacteriology·Sonali DhindwalPravindra Kumar
Jul 20, 2000·The Journal of Biological Chemistry·T WatanabeK Furukawa
Dec 17, 2002·The Journal of General and Applied Microbiology·Kensuke Furukawa
Mar 30, 2018·International Journal of Systematic and Evolutionary Microbiology·Nobutada KimuraJun Hirose
Apr 18, 2002·FEMS Microbiology Letters·Kazumasa FukudaHatsumi Taniguchi
Apr 20, 2001·The Journal of Biological Chemistry·H SuenagaK Furukawa
Dec 23, 2004·Applied Microbiology and Biotechnology·Dietmar H Pieper
Aug 5, 2004·Journal of Bacteriology·Kensuke FurukawaMasatoshi Goto
Jan 3, 2001·Applied and Environmental Microbiology·D SpringaelM Mergeay
Jun 12, 2002·Journal of Bacteriology·Hikaru SuenagaKensuke Furukawa
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·E DíazJ L García
Nov 18, 2003·Journal of Bacteriology·Marco ZielinskiBernd Hofer
Jun 29, 2005·Applied Microbiology and Biotechnology·Shin-Ichi HiranoShigenori Kanaya
Dec 3, 1998·Applied and Environmental Microbiology·A C LaytonG S Sayler
Sep 2, 2003·Journal of Bacteriology·Yong Ge, Lindsay D Eltis
Apr 3, 2007·Applied Microbiology and Biotechnology·Kazutoshi ShindoNorihiko Misawa
Feb 27, 2007·Applied and Environmental Microbiology·Beatriz CámaraBernd Hofer
Mar 5, 2005·Applied and Environmental Microbiology·Claire BagnérisJeremy R Mason

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