PMID: 9427282Jan 14, 1998Paper

Functional analyses of mammalian protein kinase C isozymes in budding yeast and mammalian fibroblasts

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
S NomotoK Irie

Abstract

The PKC1 gene of Saccharomyces cerevisiae encodes a homologue of mammalian protein kinase C (PKC) that is required for yeast cell growth. Pkc1 has been proposed to regulate a protein kinase cascade which includes the Bck1, Mkk1/Mkk2 and Mpk1 kinases. The functional relationship between Pkc1 and mammalian PKCs is unknown. Another signal transduction in Saccharomyces cerevisiae, the mating pheromone signalling pathway, is mediated by a heterotrimeric G protein, and causes cell cycle arrest in the G1 interval. It is not clear whether PKC is involved in this pathway. The effects of overexpression of PKCs in mammalian cells have been widely studied to analyse the function of PKCs in vivo. We isolated a human cDNA which encodes a protein kinase C type eta (PKC-eta) by complementation of pkc1 mutations in Saccharomyces cerevisiae. The human PKC-eta was able to complement the growth defect caused by the deletion of PKC1, whereas PKC-eta was unable to suppress the defect caused by deletion of BCK1. We also isolated human cDNAs that can suppress the adaptation defect of sst2. One of them encodes a protein kinase C type delta (PKC-delta). Expression of this gene in yeast stimulated an adaptation to the pheromone response. Human PKC-delta ...Continue Reading

Citations

May 6, 2014·Nucleic Acids Research·María Soriano-CarotJ Carlos Igual
Feb 20, 2019·Cancers·Alakananda Basu
Jan 25, 2008·The Biochemical Journal·Estela Jacinto, Anja Lorberg
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Nov 28, 2001·Gene·S M Gasser, M M Cockell
Aug 22, 2006·Biochimica Et Biophysica Acta·Maria L GasparSusan A Henry

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