Functional Analyses of the RsmY and RsmZ Small Noncoding Regulatory RNAs in Pseudomonas aeruginosa

Journal of Bacteriology
Kayley H JanssenTimothy L Yahr

Abstract

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen with distinct acute and chronic virulence phenotypes. Whereas acute virulence is typically associated with expression of a type III secretion system (T3SS), chronic virulence is characterized by biofilm formation. Many of the phenotypes associated with acute and chronic virulence are inversely regulated by RsmA and RsmF. RsmA and RsmF are both members of the CsrA family of RNA-binding proteins and regulate protein synthesis at the posttranscriptional level. RsmA activity is controlled by two small noncoding regulatory RNAs (RsmY and RsmZ). Bioinformatic analyses suggest that RsmY and RsmZ each have 3 or 4 putative RsmA binding sites. Each predicted binding site contains a GGA sequence presented in the loop portion of a stem-loop structure. RsmY and RsmZ regulate RsmA, and possibly RsmF, by sequestering these proteins from target mRNAs. In this study, we used selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) chemistry to determine the secondary structures of RsmY and RsmZ and functional assays to characterize the contribution of each GGA site to RsmY/RsmZ activity. Our data indicate that RsmA has two preferential bind...Continue Reading

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Citations

Feb 28, 2018·Journal of Bacteriology·Patricia A Champion, Joshua D Shrout
Sep 5, 2019·Journal of Bacteriology·Josh S SharpSimon L Dove
Apr 24, 2019·Journal of Bacteriology·Emily A Williams McMackinTimothy L Yahr
May 27, 2020·PLoS Pathogens·William CenensChuck S Farah
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Apr 13, 2019·International Journal of Molecular Sciences·Anjali Y BhagirathKangmin Duan
Jul 3, 2020·Frontiers in Molecular Biosciences·Juan José González Plaza
Nov 7, 2019·Scientific Reports·Marie-Francoise Noirot-GrosPhilippe Noirot
Jan 8, 2021·RNA Biology·María-Dolores FerreiroMaría-Trinidad Gallegos
Nov 8, 2020·Applied and Environmental Microbiology·Shannon R ColemanRobert E W Hancock
Mar 12, 2021·Frontiers in Molecular Biosciences·Óscar Huertas-RosalesManuel Espinosa-Urgel
Jun 30, 2021·Current Microbiology·Shuyi HouXiuyu Song
May 18, 2020·Microbial Pathogenesis·S L Rajasekhar KarnaKai P Leung
Aug 28, 2021·International Journal of Molecular Sciences·Petra PusicUdo Bläsi

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