Abstract
We identified two syntaxin-like SNARE genes, named GzSYN1 and GzSYN2, from the plant pathogenic ascomycete Gibberella zeae, and characterized the functions and cellular localization of these genes. The GzSYN1 deletion mutant (Deltagzsyn1) had 71% reduced hyphal growth compared to the wild-type strain, but produced perithecia with normal ascospores. Deltagzsyn2 had the same hyphal growth rate as the wild-type, but completely lost both self and female fertility. When Deltagzsyn2 was spermatized for Deltamat1-1 or Deltamat1-2 strains, it retained its male fertility, but the ascus shape was abnormal and ascospore delimitation was delayed. The Deltagzsyn1 and Deltagzsyn2 virulence on barley was reduced by 67% and 75%, respectively, compared to the wild-type. The GFP::GzSYN1 fusion protein was localized in vesicles, vacuoles, plasma membranes, and septa, whereas GFP::GzSYN2 was found only in plasma membranes and septa. These results suggest that syntaxins have key roles in fungal development and virulence in G. zeae.
References
Sep 1, 1987·Molecular and Cellular Biology·B G TurgeonO C Yoder
Mar 11, 1999·Fungal Genetics and Biology : FG & B·B A HorwitzB G Turgeon
Feb 5, 2000·Science·C J RobertsS H Friend
Apr 20, 2001·Molecular Plant-microbe Interactions : MPMI·F NamikiT Tsuge
Jun 8, 2002·Fungal Genetics and Biology : FG & B·Gagan D Gupta, I Brent Heath
Sep 26, 2003·Molecular Microbiology·Jungkwan LeeB Gillian Turgeon
Nov 6, 2003·Fungal Genetics and Biology : FG & B·Gagan D GuptaI Brent Heath
Jan 28, 2004·Cell·Juan S Bonifacino, Benjamin S Glick
Sep 21, 2004·Current Genetics·You-Kyoung HanYin-Won Lee
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Jae-Hyuk YuClaudio Scazzocchio
May 17, 2005·Biochimica Et Biophysica Acta·Wanjin Hong
Nov 12, 2005·Current Genetics·Vincent PhalipJean-Marc Jeltsch
Dec 13, 2005·Quarterly Reviews of Biophysics·Axel T Brunger
Feb 7, 2006·Mycologia·Steven D Harris
Feb 10, 2006·Eukaryotic Cell·Jun-ya ShojiKatsuhiko Kitamoto
Mar 24, 2006·Journal of Cell Science·Hideki NakanishiAaron M Neiman
Jun 26, 2007·Fungal Genetics and Biology : FG & B·Masahiro KuratsuKatsuhiko Kitamoto
May 27, 2008·Eukaryotic Cell·Jungkwan LeeRobert L Bowden
Jun 24, 2008·Fungal Genetics and Biology : FG & B·Hee-Kyoung KimSung-Hwan Yun
Mar 24, 2009·Applied and Environmental Microbiology·Jungkwan LeeYin-Won Lee
Jun 1, 2006·Mycotoxin Research·J F Leslie, B A Summerell
Citations
Aug 13, 2011·Current Genetics·Seunghoon LeeYin-Won Lee
Aug 24, 2012·Molecular Plant-microbe Interactions : MPMI·Kyunghun MinYin-Won Lee
Aug 31, 2010·Eukaryotic Cell·Kyunghun MinYin-Won Lee
Jun 15, 2011·Eukaryotic Cell·Seunghoon LeeYin-Won Lee
Oct 11, 2011·PloS One·Yang LinYin-Won Lee
Sep 17, 2013·PloS One·Hokyoung SonYin-Won Lee
Oct 27, 2011·PLoS Pathogens·Hokyoung SonYin-Won Lee
Oct 4, 2014·The Journal of Microbiology·Hokyoung SonYin-Won Lee
Sep 24, 2013·Current Genetics·Jungheon YuYin-Won Lee
Aug 2, 2014·Molecular Plant-microbe Interactions : MPMI·Yoonji LeeYin-Won Lee
Apr 17, 2015·Molecular Plant Pathology·Haifeng ZhangZhengguang Zhang
Nov 26, 2011·Fungal Biology·Hokyoung SonYin-Won Lee
Jan 18, 2011·Fungal Genetics and Biology : FG & B·Hokyoung SonYin-Won Lee
Sep 15, 2010·Biochemical and Biophysical Research Communications·Jungkwan LeeYin-Won Lee
Apr 21, 2012·Microbiology·Jungkwan LeeYin-Won Lee
Jun 4, 2015·Molecular Microbiology·Ae Ran ParkYin-Won Lee
Jan 7, 2014·Biotechnology Advances·Zongyi GengJinkui Yang
Jul 23, 2015·BMC Genomics·Robert KingKim E Hammond-Kosack
May 15, 2012·Microbiological Research·Hokyoung SonYin-Won Lee
Oct 13, 2015·Molecular Plant Pathology·Mira OhYin-Won Lee
Aug 28, 2010·Molecular Plant-microbe Interactions : MPMI·Yang WangJin-Rong Xu
Apr 25, 2017·Molecular Plant-microbe Interactions : MPMI·Bing LiZhengguang Zhang
Dec 13, 2017·Frontiers in Plant Science·Kihyun KimJin-Cheol Kim
Jan 28, 2014·Eukaryotic Cell·Kyunghun MinYin-Won Lee
Nov 5, 2013·Eukaryotic Cell·Hokyoung SonYin-Won Lee
Aug 8, 2019·Microbiology·Huan ZhangWon Bo Shim
Nov 2, 2019·Current Genetics·Muhammad AdnanGuo-Dong Lu
Jan 23, 2016·PloS One·Duc-Cuong BuiYin-Won Lee
Jun 3, 2020·Molecular Plant-microbe Interactions : MPMI·Sean P O'MaraH Corby Kistler
Oct 8, 2014·The Plant Pathology Journal·Boknam JungJungkwan Lee
May 9, 2019·PLoS Pathogens·Bing LiHaifeng Zhang
Mar 9, 2019·Environmental Microbiology·Bing LiShou-Qiang Ouyang
Feb 4, 2021·Scientific Reports·Wei WangGuoshu Gong
Oct 22, 2019·The Plant Pathology Journal·Taiying LiJungkwan Lee