Functional analyses of two syntaxin-like SNARE genes, GzSYN1 and GzSYN2, in the ascomycete Gibberella zeae

Fungal Genetics and Biology : FG & B
Sae-Yeon HongYin-Won Lee

Abstract

We identified two syntaxin-like SNARE genes, named GzSYN1 and GzSYN2, from the plant pathogenic ascomycete Gibberella zeae, and characterized the functions and cellular localization of these genes. The GzSYN1 deletion mutant (Deltagzsyn1) had 71% reduced hyphal growth compared to the wild-type strain, but produced perithecia with normal ascospores. Deltagzsyn2 had the same hyphal growth rate as the wild-type, but completely lost both self and female fertility. When Deltagzsyn2 was spermatized for Deltamat1-1 or Deltamat1-2 strains, it retained its male fertility, but the ascus shape was abnormal and ascospore delimitation was delayed. The Deltagzsyn1 and Deltagzsyn2 virulence on barley was reduced by 67% and 75%, respectively, compared to the wild-type. The GFP::GzSYN1 fusion protein was localized in vesicles, vacuoles, plasma membranes, and septa, whereas GFP::GzSYN2 was found only in plasma membranes and septa. These results suggest that syntaxins have key roles in fungal development and virulence in G. zeae.

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Citations

Aug 24, 2012·Molecular Plant-microbe Interactions : MPMI·Kyunghun MinYin-Won Lee
Nov 26, 2011·Fungal Biology·Hokyoung SonYin-Won Lee
Jan 18, 2011·Fungal Genetics and Biology : FG & B·Hokyoung SonYin-Won Lee
Sep 15, 2010·Biochemical and Biophysical Research Communications·Jungkwan LeeYin-Won Lee
Jun 4, 2015·Molecular Microbiology·Ae Ran ParkYin-Won Lee
Jul 23, 2015·BMC Genomics·Robert KingKim E Hammond-Kosack
Aug 28, 2010·Molecular Plant-microbe Interactions : MPMI·Yang WangJin-Rong Xu
Jun 3, 2020·Molecular Plant-microbe Interactions : MPMI·Sean P O'MaraH Corby Kistler

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