Functional analysis and heterologous expression of bifunctional glutathione synthetase from Lactobacillus

Journal of Dairy Science
Zhi-Qiang XiongLian-Zhong Ai

Abstract

Bifunctional glutathione synthetase (GshF) has recently been reported to simultaneously catalyze the 2-step ATP-dependent biosynthesis of reduced glutathione (GSH). In this work, 19 putative gshF were mined from the complete sequenced genome of 20 representative Lactobacillus species. To functionally analyze these putative GshF, GshF from Lactobacillus plantarum and Lactobacillus casei were selected and successfully expressed in Escherichia coli. Compared with the control without expressing GshF, GSH titers were enhanced significantly in E. coli with overexpression of GshF, demonstrating that putative GshF from Lactobacillus have functional activities on GSH biosynthesis. Moreover, with the expression of GshF from L. plantarum in E. coli as a paradigm, GSH yield (286.5 μM) was strongly improved by 177.9% with optimized induced conditions and precursor concentration compared with the control under unoptimized conditions. Transcriptional analysis showed that key genes of endogenous GSH metabolism and precursor biosynthesis were remarkably suppressed by GshF expression, indicating that the increase of GSH titer was attributed to heterologous expression of GshF. Overall, our results suggested that gshF is enriched in Lactobacillus ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 7, 2002·Genome Biology·Shelley D Copley, Jasvinder K Dhillon
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
Oct 14, 2004·Applied Microbiology and Biotechnology·Yin LiJian Chen
Oct 19, 2004·Applied Microbiology and Biotechnology·Yin LiDouwe Molenaar
Dec 13, 2005·The Journal of Biological Chemistry·Bjorn VergauwenJozef J Van Beeumen
Jan 21, 2009·Bioprocess and Biosystems Engineering·Liwen FeiShaoxin Chen
May 24, 2011·Journal of Bioscience and Bioengineering·Takahiro SuzukiSatoshi Yoshida
Dec 14, 2011·Journal of Bacteriology·Roland J SiezenSacha A F T van Hijum
Jan 12, 2012·Applied Microbiology and Biotechnology·Kiyotaka Y HaraAkihiko Kondo
May 23, 2012·Applied and Environmental Microbiology·Shaolin GeYin Li
Aug 28, 2012·Microbial Cell Factories·Sarang Dilip PophalySudhir Kumar Tomar
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 21, 2015·Journal of Industrial Microbiology & Biotechnology·Dezheng WangQin Ye

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Citations

Sep 15, 2020·Molecular Biology Reports·Xin SongLian-Zhong Ai

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