Functional analysis of a Brassica oleracea SLR1 gene promoter

Plant Physiology
R M HackettF C Franklin

Abstract

The Brassica oleracea S-locus-related gene 1 (SLR1) is expressed in the papillar cells of Brassica stigmas from a few days before anthesis. We have previously shown that a 1500-bp fragment of the SLR1 gene promoter is sufficient to direct high-level, temporally regulated expression of the beta-glucuronidase reporter gene in the pistils of transgenic tobacco. We have carried out a deletion analysis of the SLR1 promoter and found that elements required for pistil expression are located between -258 and -327 bp (relative to the translation start site). Furthermore, specific binding of pistil nuclear factors to sequences within this region was demonstrated by gel retardation analysis. Sequences between -1350 and -1500 were found to be required for high-level expression.

Citations

Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Paul M. HasegawaHans J. Bohnert
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Elizabeth H Harris
Jul 21, 2004·Journal of Molecular Microbiology and Biotechnology·Torsten von RozyckiMilton H Saier
Apr 17, 2007·The FEBS Journal·Catarina PimentelClaudina Rodrigues-Pousada
Jan 17, 2020·Journal of Experimental Botany·Frédérique RozierIsabelle Fobis-Loisy
Mar 3, 2021·The Plant Journal : for Cell and Molecular Biology·Reneé RobinsonLaurian S Robert

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