PMID: 7535867May 1, 1995Paper

Functional analysis of amino acid residues encompassing and surrounding two neighboring H-2Db-restricted cytotoxic T-lymphocyte epitopes in simian virus 40 tumor antigen

Journal of Virology
J D LippolisS S Tevethia

Abstract

Simian virus 40 tumor (T) antigen contains three H-2Db-and one H-2Kb-restricted cytotoxic T lymphocyte (CTL) epitopes (sites). Two of the H-2Db-restricted CTL epitopes, I and II/III, are separated by 7 amino acids in the amino-terminal one third of T antigen. In this study, we determine if the amino acids separating these two H-2Db-restricted CTL epitopes are dispensable for efficient processing and presentation. In addition, the importance of amino acid residues lying within and flanking the H-2Db-restricted epitopes I and II/III for efficient processing, presentation, and recognition by site-specific CTL clones was determined by using T-antigen mutants containing single-amino-acid substitutions between residues 200 and 239. Using synthetic peptides in CTL lysis and major histocompatibility complex class I stabilization assays, CTL recognition site I has been redefined to include residues 206 to 215. Substitutions in amino acids flanking either site I or site II/III did not affect recognition by any of the T-antigen-specific CTL clones. Additionally, the removal of the 7 residues separating site I and site II/III did not affect CTL recognition, thus demonstrating that these two epitopes when arranged in tandem in the native T ...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M WiglerL Chasin
Feb 1, 1992·The Journal of Experimental Medicine·L C EisenlohrJ R Bennink
Sep 26, 1991·Nature·T S JardetzkyD C Wiley
Jul 27, 1990·Cell·A TownsendA Tse
Aug 2, 1990·Nature·H G LjunggrenK Kärre
Dec 1, 1985·The Journal of Experimental Medicine·H G Ljunggren, K Kärre
Apr 1, 1973·Virology·F L Graham, A J van der Eb

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Citations

Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T D Schell, S S Tevethia
Aug 1, 1997·Immunology and Cell Biology·A Suhrbier
Dec 25, 2007·Journal of Animal Science·J D Lippolis
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pavel OtahalTodd D Schell
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christina M Ryan, Todd D Schell

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