Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression.

The Plant Cell
Yoh SakumaKazuko Yamaguchi-Shinozaki

Abstract

Transcription factors DREB1A/CBF3 and DREB2A specifically interact with cis-acting dehydration-responsive element/C-repeat (DRE/CRT) involved in cold and drought stress-responsive gene expression in Arabidopsis thaliana. Intact DREB2A expression does not activate downstream genes under normal growth conditions, suggesting that DREB2A requires posttranslational modification for activation, but the activation mechanism has not been clarified. DREB2A domain analysis using Arabidopsis protoplasts identified a transcriptional activation domain between residues 254 and 335, and deletion of a region between residues 136 and 165 transforms DREB2A to a constitutive active form. Overexpression of constitutive active DREB2A resulted in significant drought stress tolerance but only slight freezing tolerance in transgenic Arabidopsis plants. Microarray and RNA gel blot analyses revealed that DREB2A regulates expression of many water stress-inducible genes. However, some genes downstream of DREB2A are not downstream of DREB1A, which also recognizes DRE/CRT but functions in cold stress-responsive gene expression. Synthetic green fluorescent protein gave a strong signal in the nucleus under unstressed control conditions when fused to constitut...Continue Reading

References

Oct 25, 1990·Nucleic Acids Research·T D Schneider, R M Stephens
Aug 18, 1988·Nature·J MaM Ptashne
Jul 1, 1996·Trends in Biochemical Sciences·M Rechsteiner, S W Rogers
Dec 1, 1996·The Plant Journal : for Cell and Molecular Biology·T UraoK Shinozaki
Sep 15, 1998·Biochemical and Biophysical Research Communications·Z K ShinwariK Shinozaki
Jul 16, 1999·The Plant Journal : for Cell and Molecular Biology·Y NiwaH Kobayashi
Jan 19, 2002·Biochemical and Biophysical Research Communications·Yoh SakumaKazuko Yamaguchi-Shinozaki
Jul 2, 2002·Plant Molecular Biology·Ming-Jun GaoJas Singh
Aug 15, 2002·Plant Physiology·Dong-Woog ChoiTimothy J Close
Sep 12, 2002·Annual Review of Plant Biology·Jian-Kang Zhu
Oct 12, 2002·Plant Physiology·Volker HaakeJames Z Zhang
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Joseph G DubouzetKazuko Yamaguchi-Shinozaki
Sep 16, 2003·Current Opinion in Plant Biology·Kazuo ShinozakiMotoaki Seki
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Michael F. Thomashow
May 29, 2004·The Plant Journal : for Cell and Molecular Biology·Kyonoshin MaruyamaKazuko Yamaguchi-Shinozaki
Feb 15, 2005·Trends in Plant Science·Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki

❮ Previous
Next ❯

Citations

Jul 22, 2006·Plant Cell Reports·Pradeep K AgarwalSudhir K Sopory
Nov 21, 2007·Plant Cell Reports·Pooja Bhatnagar-MathurKiran K Sharma
Mar 12, 2009·Molecular Genetics and Genomics : MGG·Lam-Son Phan TranHenry T Nguyen
Sep 21, 2011·Molecular Genetics and Genomics : MGG·Takumi YoshidaKazuko Yamaguchi-Shinozaki
Apr 25, 2009·Molecules and Cells·Sun-ji LeeSoo Young Kim
Jun 29, 2010·Functional & Integrative Genomics·Lam-Son Phan Tran, Keiichi Mochida
Sep 8, 2010·Functional & Integrative Genomics·Swatismita RayAkhilesh K Tyagi
May 5, 2009·Journal of Plant Research·Motoaki Seki, Kazuo Shinozaki
Mar 19, 2011·Journal of Plant Research·Yasunari FujitaKazuko Yamaguchi-Shinozaki
Aug 30, 2011·Journal of Plant Research·Yuriko OsakabeKeishi Osakabe
Sep 10, 2008·Biotechnology Letters·Jian-Qiang ChenXi-Ping Wang
Aug 9, 2007·Plant Molecular Biology·Anwesha NagThomas Jack
Oct 31, 2012·Plant Molecular Biology·Shuxin ZhangPieter B F Ouwerkerk
Mar 10, 2015·Journal of Advanced Research·Nemat M HassanMamdouh M Nemat Alla
Oct 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hongbo GaoRobert M Larkin
May 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nils ElfvingStefan Björklund
Sep 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Daisuke TodakaKazuko Yamaguchi-Shinozaki
Nov 11, 2009·The Journal of Biological Chemistry·Kohji YamadaKazuko Yamaguchi-Shinozaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.