Functional analysis of Arabidopsis V-ATPase subunit VHA-E isoforms

European Journal of Cell Biology
Jan DettmerKarin Schumacher

Abstract

Acidification of endomembrane compartments by the vacuolar H(+)-ATPase (V-ATPase) is an important mechanism to generate microenvironments suitable for various cellular functions. Differential assembly of subunit isoforms provides the potential to flexibly adapt the proton-pumping V-ATPase complex to changing physiological conditions and cell type-specific requirements. In Arabidopsis, the regulatory V-ATPase subunit E (VHA-E) is encoded by three genes with distinct expression patterns. We show here that VHA-E2, which belongs to a clade of pollen-specific VHA-E isoforms present in all higher plants, has a specialized but non-essential function during gametophyte development. Similarly, loss of the epidermis-specific isoform VHA-E3, which we show here to be transcriptionally regulated by the phytohormone jasmonic acid, does not cause obvious phenotypic changes. Furthermore, when expressed ubiquitously, VHA-E3, in contrast to VHA-E2, is able to complement loss of the essential subunit VHA-E1 indicating different degrees of functional specialization among the Arabidopsis VHA-E isoforms.

References

Feb 29, 2000·The Journal of Biological Chemistry·Y KawamuraS Yoshida
Dec 7, 2002·The Plant Cell·Allen SessionsStephen A Goff
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Jul 22, 2004·Plant Physiology·Iris TzafrirDavid Meinke
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Apr 5, 2005·Nature Genetics·Markus SchmidJan U Lohmann
Jun 16, 2006·The Journal of Biological Chemistry·Masashi OhiraPatricia M Kane
Oct 4, 2007·Nature Reviews. Molecular Cell Biology·Michael Forgac
Oct 29, 2008·The Journal of Biological Chemistry·Zhenyu ZhangStephan Wilkens

❮ Previous
Next ❯

Citations

Feb 18, 2011·Plant Physiology·Yoichiro FukaoMasayoshi Maeshima
Aug 19, 2011·Plant Signaling & Behavior·Yoichiro Fukao, Ali Ferjani
Feb 11, 2015·Plant Physiology·Simon PearceZoe A Wilson

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.