Functional analysis of bispecific antibody (EpCAMxCD3)-mediated T-lymphocyte and cancer cell interaction by single-cell force spectroscopy

International Journal of Cancer. Journal International Du Cancer
Sabrina C HoffmannThomas Ludwig

Abstract

The atomic force microscopy (AFM) is a powerful tool to analyze forces generated on cellular interactions on the single-cell level. This highly sensitive device can record changes in force in the pico-Newton range, which equals single molecule bonds. Here, we have used single-cell force spectroscopy by AFM to investigate the interaction between T cells and tumor cells that is induced by the bispecific antibody HEA125xOKT3 (specificity anti-EpCAMxCD3). We show that HEA125xOKT3 induces a specific increase in adhesion force between T cells and cancer cells. The adhesive force that is generated on cell-cell contact is dependent on the applied force on initial contact and the duration of this initial contact. In summary, HEA125xOKT3 has been found to mediate contact formation by distinct processes. It induces direct cell-cell interaction, which results in the activation of T-cell signaling, facilitates the formation of supramolecular activation clusters and ultimately of an immune synapse.

References

Oct 1, 1995·Immunology Today·M LubC G Figdor
Mar 3, 1986·Physical Review Letters·G BinnigC Gerber
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Alexander MarméGerhard Moldenhauer
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Jan 28, 2004·Human Pathology·Philip Th WentStephan Dirnhofer
Mar 6, 2004·Seminars in Immunology·Jane C Stinchcombe, Gillian M Griffiths
Jan 18, 2006·Experimental Hematology·Lawrence G LumRandall J Lee
Feb 7, 2006·Journal of Immunological Methods·M H KonstandinY Samstag
Dec 1, 2006·Annual Review of Immunology·Gabriel A RabinovichEduardo M Sotomayor
Feb 7, 2007·Cancer Research·Chenwei LiDiane M Simeone
May 15, 2007·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Nicolas Fischer, Olivier Léger
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Piero DalerbaMichael F Clarke
Oct 4, 2007·Science's STKE : Signal Transduction Knowledge Environment·Clemens M FranzDaniel J Muller
Dec 7, 2007·Pflügers Archiv : European journal of physiology·Thomas LudwigUlrich S Schwarz
May 22, 2008·Journal of Cell Science·Jonne HeleniusDaniel J Muller
Jun 10, 2008·Journal of Immunological Methods·Kathleen E McGrathJames Palis
Jul 8, 2008·Biochemical and Biophysical Research Communications·Mingqian HuXiaoping Wang
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Babak H HosseiniGünter J Hämmerling

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Citations

Mar 19, 2013·The International Journal of Biochemistry & Cell Biology·H M SchröderT Ludwig
Sep 22, 2017·Journal of Hematology & Oncology·Shengnan YuKongming Wu
Oct 23, 2012·Nanotechnology·Govind PaneruBret N Flanders
Aug 24, 2017·Pharmacology & Therapeutics·Z Wu, N V Cheung
Jun 7, 2021·Experimental Cell Research·Khadijeh BarzamanLeila Farahmand

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