Functional analysis of carp interferon-gamma: evolutionary conservation of classical phagocyte activation

Fish & Shellfish Immunology
J A J ArtsB M Lidy Verburg-van Kemenade

Abstract

In teleost fish two IFN-gamma gene sequences were found for which two phylogenetic clusters can be distinguished. Our previous analysis of expression of these in carp led us to hypothesize that a classical IFN-gamma function is associated with the IFN-gamma2 cluster. We investigated the evolutionary conservation of the IFN-gamma function, inducing classical activation of phagocytes, thus skewing towards a Th1-like profile of immune activation. Recombinant proteins for the carp IFN-gamma sequences of both clusters were made and we studied their effects on expression of proinflammatory mediators. Carp IFN-gamma2, in contrast to carp IFN-gamma1, was powerful in inducing a proinflammatory reaction in phagocytes: a classical synergistic response with lipopolysaccharide was observed for the induction of iNOS expression and NO release, for expression of CXCL9-11-like chemokines and the expression of proinflammatory cytokines IL-1beta, TNFalpha and the IL-12 subunits p35 and p40. In contrast, like in mammals, the CXCL8-like cytokines are LPS but not IFN-gamma sensitive. These results corroborate an evolutionary conserved nature of IFN-gamma function in lower vertebrates including classical activation of phagocytes.

Citations

Sep 17, 2013·Fish & Shellfish Immunology·Tiehui Wang, Christopher J Secombes
Aug 11, 2011·Virology Journal·Astrid SkjesolJorunn B Jørgensen
Jul 19, 2014·Developmental and Comparative Immunology·M ChadzinskaB M L Verburg-van Kemenade
Dec 29, 2011·Journal of Signal Transduction·Tiehui WangChristopher J Secombes
Dec 4, 2015·Biology·Jordan W HodgkinsonMiodrag Belosevic
Jun 19, 2012·Developmental and Comparative Immunology·Lieke M van der AaB M Lidy Verburg-van Kemenade
Jul 26, 2011·Developmental and Comparative Immunology·Jun Zou, Christopher J Secombes
Jun 7, 2011·Developmental and Comparative Immunology·Magdalena ChadzinskaB M Lidy Verburg-van Kemenade
May 25, 2011·Developmental and Comparative Immunology·C J SecombesS Bird
Mar 19, 2011·Developmental and Comparative Immunology·Kerry J Laing, John D Hansen
Mar 19, 2011·Developmental and Comparative Immunology·Maria ForlenzaGeert F Wiegertjes
Mar 19, 2011·Developmental and Comparative Immunology·Aja M Rieger, Daniel R Barreda
Jan 26, 2011·General and Comparative Endocrinology·B M L Verburg-van KemenadeM Chadzinska
Oct 17, 2015·Molecular Immunology·Geert F WiegertjesInge R Fink
Dec 21, 2011·Developmental and Comparative Immunology·Lieke M van der AaB M Lidy Verburg-van Kemenade
Oct 25, 2011·Developmental and Comparative Immunology·Aina-Cathrine OvergårdSonal Patel
Dec 4, 2012·General and Comparative Endocrinology·B M L Verburg-van KemenadeM Chadzinska
May 10, 2016·Developmental and Comparative Immunology·Yasuhiro ShibasakiTeruyuki Nakanishi
May 28, 2016·Biology·Jun Zou, Christopher J Secombes
Aug 20, 2016·Fish & Shellfish Immunology·Sohye YoonChristopher J Secombes
Nov 5, 2016·Fish & Shellfish Immunology·Patricia PereiroBeatriz Novoa
Nov 13, 2015·Biology·Jeffrey J Havixbeck, Daniel R Barreda
Jun 13, 2018·Frontiers in Immunology·Leon GrayferMiodrag Belosevic
Jun 11, 2020·Fish Physiology and Biochemistry·Magdalena MaciuszekMagdalena Chadzinska
May 10, 2020·Environmental Science and Pollution Research International·Mehwish FaheemAlaa El-Din-H-Sayed
Aug 12, 2020·Scientific Reports·Annelieke S WentzelGeert F Wiegertjes
Mar 12, 2020·Frontiers in Immunology·Annelieke S WentzelGeert F Wiegertjes
Dec 2, 2020·International Journal of Molecular Sciences·Magdalena MaciuszekMagdalena Chadzinska
Oct 20, 2019·Developmental and Comparative Immunology·Lukasz PijanowskiMagdalena Chadzinska
Oct 11, 2020·Fish & Shellfish Immunology·Magdalena MaciuszekMagdalena Chadzińska
Jun 4, 2021·Developmental and Comparative Immunology·Hongxiang WangWenbin Zhan

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